such as "Introduction", "Conclusion"..etc
Figure 1 Overview of three types of Drosophila neurons. (a) Larval mushroom body interneurons. These neurons were marked by crossing flies containing the mushroom body Gal4 driver 201Y with flies containing a UAS-mCD8-GFP transgene. Brains were dissected from third instar larval progeny. A projection of a confocal image stack is shown. The structure is shown schematically to the right, and a diagram of a single neuron is shown at the lower right. (b) Olfactory projection interneurons. These neurons were marked by crossing GH146 Gal4 and UAS-mCD8-GFP flies. Brains from third instar progeny were dissected and a projection of a confocal stack is shown. The GH146 Gal4 driver also expresses in the optic lobe, which accounts for the bright GFP in the lateral region of the brain. Different compartments of the projection neurons are outlined. The axon projections are outlined to their synapses on the mushroom body calyx, but the continuation to the lateral horn is not outlined as the axons become more difficult to follow. (c) Sensory neurons in the dorsal cluster, including dendritic arborization neurons. Neurons were labeled by crossing elav-Gal4 and UAS-mCD8-GFP flies. Confocal microscopy was used to image just below the cuticle of whole, live early second instar progeny; a projection is shown with the scale inverted for clarity. Cell bodies are in the center of the image. Anterior is left and dorsal is up. Dendrites project dorsally and branch extensively just under the cuticle. Axons project down and form a tight bundle.
Figure 2 Mushroom body neurons are divided into molecularly distinct compartments. Mushroom body neurons expressing tagged markers were generated by crossing flies containing mushroom body Gal4 drivers with flies carrying UAS-controlled markers. The 201Y Gal4 was used in all panels except L10-YFP and homer-GFP, for which the OK107 Gal4 was used. 201Y drives expression in a large subset of mushroom body neurons [54, 55], and OK107 drives expression in all mushroom body neurons  as well as the optic lobe. Brains were dissected from third instar larvae, fixed and analyzed by confocal microscopy. All panels represent confocal projections through the entire mushroom body, except homer-GFP, which shows the cell bodies, dendrites and proximal axons, with distal axons in the inset. Low expression levels of n-syb-YFP expression were generated by using a 2XUAS vector, and low levels of homer-GFP were generated by raising larvae at 18°C. Arrow points to region of proximal axon in which Apc-2 GFP was localized. Scale bar at lower right is 20 μm.
Figure 3 Olfactory projection neurons have molecularly distinct compartments. Tagged markers were expressed in olfactory projection neurons using the GH146 Gal4 driver, which is specific to these neurons and a few other scattered groups of neurons in the brain . Brains were dissected from third instar larvae and fixed. Signal in nod-YFP and NgCAM-YFP panels was amplified by staining with GFP antibodies. Confocal projections through the region occupied by projection neurons are shown. The arrow in the nod-YFP panel represents fluorescence from other neurons; the asterisk indicates the projection neuron axons terminate; arrowheads indicate the beginning of the axons, just distal to the dendrite branch point. Scale bar is 20 μm.
Figure 4 Tagged endogenous proteins localize to distinct neuronal compartments. Homozygous fly lines containing GFP transposon insertions were imaged by confocal microscopy. Single confocal sections of third instar brains are shown. The images were all acquired at the depth of the mushroom body calyx; the calyx region is indicated in each image with an asterisk. A diagram of a section through a third instar brain is shown at the upper left. One brain lobe (boxed region) is shown in each panel. At the top right an example of a GFP transposon insertion that yields cytoplasmic fluorescence in the cell body, axons and dendrites is shown. The bel, Pdi, and Map205 genes all contain Wee-P  insertions, and the Jupiter line was previously described . Scale bar at lower left is 50 μm.
Figure 5 Determination of microtubule polarity in axons and dendrites using EB1-GFP dynamics. Flies containing an elav-Gal4 transgene were crossed to flies with a UAS-EB1-GFP transgene. Progeny were aged to early L2 and whole larvae were mounted for imaging. Single confocal images were acquired every 3.6 seconds. Additional data file 1 is a movie that shows all images acquired. Frames from two portions of the movie were selected for still images. The top row shows EB1-GFP fluorescence in an axon of a dendritic arborization neuron. The arrow and arrowhead track two different EB1-GFP dots as they move away from the cell body. The bottom row shows the dendrite from the same neuron as the top panels; these images were acquired at a different plane of focus. Three different EB1-GFP dots are tracked. The one indicated with the upward-pointing arrow moves away from the cell body and the other two move towards the cell body. The figure is shown with anterior up and dorsal to the left. Scale bar is 5 μm.
Figure 6 The n-syb-YFP marker is localized to synaptic vesicles in mushroom body dendrites. Brains from third instar larvae expressing n-syb-YFP in mushroom body neurons were dissected and immunostained for CSP and Scrib. A single confocal section through the mushroom body calyx is shown. The arrows show clusters of n-syb-YFP dots that colocalize with CSP. The asterisk shows a region that has Scrib and CSP staining, but no n-syb-YFP. Scale bar is 5 μm.
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