such as "Introduction", "Conclusion"..etc
Arsenic is a toxic, naturally occurring element that is sometimes found at high concentrations in well water. High arsenic concentrations can occur even in areas without a pollution source when the bedrock or soil that is in contact with the ground water releases arsenic as the water flows over the surfaces.
Iron reducing bacteria, such as members of the genus Geobacter, grow in the absence of oxygen and can transform solid phase iron (Fe(III)) into Fe(II), which is soluble in water. Solid phase Fe(III) can bind to arsenic, immobilizing it on the surface of the solid. When bedrock or soil Fe(III) is transformed into Fe(II), any bound arsenic would also be released into the groundwater. Arsenic (As) also has two commonly occurring forms in groundwater: As(III) and As(V).
The prevalence of NP4, a microbe from the genus Sulfurospirillum that can transform As(V) to As(III), which is the more toxic form of As, was also measured. NP4 was more abundant in water samples with higher As(III) concentrations. Thus it appears that iron reducing bacteria affect the overall arsenic concentration, and that arsenic reducing bacteria (NP4) control its form, and thus toxicity, in these regions of Maine.
This work was completed at the University of Maine by Ph.D. candidate Jennifer Weldon under the supervision of Dr. Jean MacRae. The project, funded by the National Science Foundation (NSF) Career program (Bioengineering and Environmental Systems), is being presented at the General Meeting of the American Society of Microbiology (ASM), June 8th 2005 in Atlanta, GA.
The 15 groundwater samples used in the study were examined using fluorescence in-situ hybridization (FISH). This technique allowed the target populations, Geobacter and NP4, to be distinguished from other bacteria. The number of each type of microorganism was compared to the total number of microorganisms in the sample. Geobacter ranged from 1-35% and NP4 from 0-17% of the total suspended bacterial population. Metals were measured by inductively coupled plasma atomic emission absorption (ICP-AES), and arsenic speciation was obtained by passing a sample through an ion exchange resin in the field to obtain As(III). Total arsenic concentrations ranged from less than 2 parts per billion (ppb) to 2000 ppb, which is 200 times higher than the EPA's water quality limit of 10 ppb. As(III) ranged from
Source: American Society For Microbiology. June 2005.
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