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<p>..................................................</p> <p><em><strong>Figure 1.</strong> Experimental design. Cartilage cells were seeded onto polymer scaffolds, and the resulting constructs were cultivated in rotating bioreactors first for 3 months on Earth and then for 4 additional months either on the Mir Space Station (10<sup><img alt="-" src="http://www.pnas.org/math/12pt/normal/scr/minus.gif">4</sup>-10<sup><img alt="-" src="http://www.pnas.org/math/12pt/normal/scr/minus.gif">6</sup> g) or on Earth (1 g). </em></p> <p><em><img alt="Figure 1" src="/user_files/Image/Bioengineering/BE-tissueengineeringspaceF01.gif"></em></p> <p>..................................................</p> <p><em><strong>Figure 2.</strong> Cell viability postflight. Cells isolated from a 7-month construct from the Mir group after 2 days of monolayer culture. (×200.) Intracellular esterase activity is indicated by green color. </em></p> <p><em><img alt="Figure 2" src="/user_files/Image/Bioengineering/BE-tissueengineeringspaceF02.gif"></em></p> <p>..................................................</p> <p><em><strong>Figure 3.</strong> Construct structure. (A and B) Full cross sections of constructs from Mir and Earth groups. (×10.) (C and D) Representative areas at the construct surfaces. (×200.) GAG is stained red with safranin-O. </em></p> <p><em><img alt="Figure 3" src="/user_files/Image/Bioengineering/BE-tissueengineeringspaceF03.gif"></em></p> <p>..................................................</p> <p><em><strong>Figure 4.</strong> Construct ultrastructure. Transmission electron micrographs of constructs from Mir (A) and Earth (B) groups at magnifications of ×3,500 and ×120,000 (Inset). </em></p> <p><em><img alt="Figure 4" src="/user_files/Image/Bioengineering/BE-tissueengineeringspaceF04.gif"></em></p> <p>..................................................</p>
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