such as "Introduction", "Conclusion"..etc
If you want to know how a cell responds to a particular chemical, the
experiment is simple: Inject it with that chemical. Micropipettes — tiny
needles that can puncture a cell and deliver a compound directly into
it — are used precisely for this purpose. But biologists who study yeast
have not had this tool available to them. A yeast cell’s rigid outer
wall is too strong to be penetrated.
Rockefeller University’s Paul Nurse, president and head of the
Laboratory of Yeast Genetics and Cell Biology, and Daniel Riveline, a
visiting professor from the French National Center for Scientific
Research (CNRS), have developed a technique that solves this problem.
They used a tiny electric motor in conjunction with a pipette to “saw”
an opening in the cell’s tough outer coating. By borrowing tools from
the microfabrication industry, the new method allows scientists to
successfully introduce markers such as fluorescent proteins into Schizosaccharomyces
pombe yeast without killing them.
Rather than use the sharp, pointed end of the micropipette, the
scientists use the broad side of its flexible tip to bend the cell wall
of a single rod-shaped yeast cell that is held in place by a
microfabricated grid-shaped substrate. After the wall buckles, they turn
on a piezoelectric motor to vibrate the pipette’s tip, opening a hole
and allowing its contents to enter the cell.
“Not all the cells survive the procedure, but a significant number
do,” says Riveline, the technique’s innovator, who began to assemble the
apparatus in his lab in the Laboratory of Physical Spectrometry
(CNRS/Grenoble University). “The cell is able to repair the hole and can
continue to grow and divide.”
Because its processes of growth and division are similar to those of
mammals, yeast is a useful model organism for cell biologists,
particularly those studying the cell cycle. The technique opens up the
possibility of new types of experiments in both S. pombe and S.
cerevisiae budding yeast, which has a similar cell wall.
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