such as "Introduction", "Conclusion"..etc
October 05, 2006 --
With the genomes of hundreds of organisms now catalogued, one of the
next major challenges is to identify proteins and their interactions.
The current issue of CSH Protocols, published online
(www.cshprotocols.org), features two freely available, cutting-edge
methods that address this challenge.
of protein-DNA interactions has been a tedious process, limited to one
protein-interaction partner at a time. But the advent of
high-throughput, genomics-based technologies has enabled scientists to
build large-scale maps of protein-DNA and protein-protein interactions.
These methods-released in CSH Protocols-will be useful to researchers
seeking to map these interactions and better understand fundamental
One of the freely accessible protocols, developed by Dr. Marian Walhout
and her colleagues at the University of Massachusetts Medical School,
describes a means for identifying protein-DNA interactions in complex
organisms. Her team has used this method to generate a protein-DNA
interaction network by characterizing the DNA sequences to which
proteins bind in the model organism C. elegans (the "worm" used in many
developmental studies). Because it relies on basic molecular biology
techniques, this method will also be useful for analyzing the
intricately coordinated patterns of gene regulation in other species.
technical challenge facing researchers is that in a typical cell,
proteins are present in relatively small quantities. The second free
method in this release of CSH Protocols is "MuDPIT" (for
Multidimensional Protein Identification Technology), a means of
identifying rare proteins in complex mixtures. This method combines
advanced protein identification technologies with computational
analysis, and has already been successfully employed in characterizing
interacting proteins in yeast.
These methods are among more than
40 new peer-reviewed protocols added to the CSH Protocols database this
month. Launched in May of 2006, CSH Protocols now includes more than
700 protocols spanning many fields in cell and molecular biology.Source : Cold Spring Harbor Laboratory
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