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<table border="0" cellspacing="5" cellpadding="0"><tbody><tr><td colspan="5"> <p> <strong>Comparison of the discussed four different enrichment approaches</strong> </p> </td> </tr><tr><td> </td> <td align="center"><strong>DGC</strong><br></td> <td align="center"><strong>RS+DGC</strong><br></td> <td align="center"><strong>DGC+MCS</strong><br></td> <td align="center"><strong>DGC+FACS</strong><br></td> </tr><tr><td colspan="5"> <hr></td> </tr><tr><td align="left"><strong>Purity</strong><br></td> <td align="center">ca. 70% <sup>a</sup><br></td> <td align="center">ca. 93% <sup>a</sup><br></td> <td align="center">> 98% <sup>d</sup><br></td> <td align="center">90 – 95% <sup>f</sup><br></td> </tr><tr><td> </td> <td align="center">CD5<sup>+</sup>/CD19<sup>+</sup><br></td> <td align="center">CD5<sup>+</sup>/CD19<sup>+</sup><br></td> <td align="center">> 93% <sup>e</sup><br></td> <td align="center">CD5<sup>+</sup>/CD19<sup>+</sup><br></td> </tr><tr><td> </td> <td> </td> <td> </td> <td align="center">CD19<sup>+</sup><br></td> <td> </td> </tr><tr><td> </td> <td align="center">86% (± 14%) <sup>b</sup><br></td> <td> </td> <td> </td> <td> </td> </tr><tr><td> </td> <td align="center">CD19<sup>+</sup><br></td> <td> </td> <td> </td> <td> </td> </tr><tr><td> </td> <td align="center">90% (± 7%) <sup>c</sup><br></td> <td> </td> <td> </td> <td> </td> </tr><tr><td> </td> <td align="center">CD5<sup>+</sup>/CD19<sup>+</sup>/CD23<sup>+</sup><br></td> <td> </td> <td> </td> <td> </td> </tr><tr><td align="left"><strong>Time of procedure</strong><br></td> <td align="center">< 60 mins.<br></td> <td align="center">< 90 mins.<br></td> <td align="center">> 120 mins (increasing with sample volume)<br></td> <td align="center">> 120 mins (increasing with sample volume)<br></td> </tr><tr><td align="left"><strong>Selection method</strong><br></td> <td align="center">negative<br></td> <td align="center">negative<br></td> <td align="center">positive<br></td> <td align="center">positive<br></td> </tr><tr><td align="left"><strong>Limitations</strong><br></td> <td align="center">highly depending on WBC count<br></td> <td align="center">dependent on WBC count when < 20 × 10<sup>6 </sup>cells/ml PB<br></td> <td align="center">long process time and expensive<br></td> <td align="center">long process time and very expensive<br></td> </tr><tr><td align="left"><strong>cost</strong><br></td> <td align="center">low<br></td> <td align="center">medium<br></td> <td align="center">medium-high<br></td> <td align="center">high<br></td> </tr><tr><td align="left"><strong>Required Infra-structure</strong><br></td> <td align="center">centrifuge<br></td> <td align="center">centrifuge<br></td> <td align="center">centrifuge + MCS Sorter<br></td> <td align="center">centrifuge + FACS sorter<br></td> </tr><tr><td colspan="5"> <hr></td> </tr><tr><td colspan="5"> <p> DGC: density gradient centrifugation, RS+DGC: RosetteSep incubation prior to DGC, DGC+MCS: DGC and subsequent magnetic cell sorting, DGC+FACS: DGC and subsequent fluorescent activated cell sorting. </p> <p> <sup>a </sup>average purity experienced in our study </p> <p> <sup>b </sup>purity reported by Haslinger et al., 2004 </p> <p> <sup>c </sup>purity reported by Falt et al., 2005 </p> <p> <sup>d </sup>purity reported by Rosenwald et al., 2004 and Wiestner et al., 2003 </p> <p> <sup>e </sup>Huttman et al., 2006 </p> <p> <sup>f </sup>purity reported by Stankovic et al., 2004 </p> </td> </tr></tbody></table>
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