such as "Introduction", "Conclusion"..etc
Gianluca Tell1, Igor Paron1 and Marcello Civilini2
Biotechnology Letters 10.1007/s10529-006-9197-1.
qualitative procedure of purified DNA/RNA co-extraction from complex
organic matter, used as biofilter support for removing volatile organic
compounds, was set up and applied to detect xylene monooxygenase gene
expression by RT-PCR. A DNA/RNA extraction protocol based on a
combination of sample lyophilization pre-treatment and
CTAB––phenol/chloroform extraction procedure was optimized for the
recovery of purified nucleic acids [100–500 ng DNA (10 kb) and 0.5–2 μg
of rRNA 16S from 100 mg matrix]. PCR and RT-PCR protocols were
established to detect xylene monooxygenase gene expression starting
from differentially induced organic matrices obtained by biofiltration
technology. This work allowed the microbial degradation activities in
heterogeneous organic solid media to be studied and suggests a rapid
method to follow specific biological activities during solid and/or
semisolid organic substrates biotransformation.
Keywords Biofiltration - Nucleic acids - Organic matter - RT-PCR - Toluene/benzene-2-monooxygenase - Xylene monooxygenase
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