Ricardo Pinto1, António L Amaral1,2, Eugénio C Ferreira1, Manuel Mota1, Manuel Vilanova3,4, Katia Ruel5 and Miguel Gama1
for Biotechnology and Bioengineering, Centre of Biological Engineering,
Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal
2Departamento de Tecnologia Química, ESTIG, IPB, Apartado 1038, 5301-854 Bragança, Portugal
3ICBAS – Instituto de Ciências Biomédicas de Abel Salazar, Largo do Professor Abel Salazar 2, 4099-003 Porto, Portugal
4IBMC – Instituto de Biologia Molecular e Celular, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal
5Centre de Recherches sur les Macromolécules Végétales (CERMAV)-UPR CNRS 5301, BP 53, 38041 Grenoble cedex 9, France
BMC Biotechnology 2008,
8: 1 [Open Access].
Cellulose Binding Domains (CBD) were conjugated with fluorescein
isothiocyanate (FITC). The surface concentration of the Binding Domains
adsorbed on cellulose fibres was determined by fluorescence image
For a CBD-FITC concentration of 60 mg/L, a coating fraction of 78% and 110% was estimated for Portucel and
Whatman fibres, respectively. For a saturating CBD concentration, using
Whatman CF11 fibres, a surface concentration of 25.2 × 10-13 mol/mm2 was
estimated, the equivalent to 4 protein monolayers. This result does not
imply the existence of several adsorbed protein layers.
It was verified that CBDs were able to penetrate the fibres,
according to confocal microscopy and TEM-immunolabelling analysis. The
surface concentration of adsorbed CBDs was greater on amorphous fibres
(phosphoric acid swollen) than on more crystalline ones (Whatman CF11
and Sigmacell 20).