Submit a Revision

*your email :**
page name* : such as "Introduction", "Conclusion"..etc
<h1>Efficient and reproducible generation of high-expressing, stable human cell lines without need for antibiotic selection</h1> <div> <p> <strong>Gudrun Schiedner</strong>, <strong>Sabine Hertel</strong>, <strong>Corinna Bialek</strong>, <strong>Helmut Kewes</strong>, <strong>Gero Waschütza</strong> and <strong>Christoph Volpers</strong> </p> <p> CEVEC Pharmaceuticals GmbH, Gottfried-Hagen-Str. 62, 51105 Cologne, Germany </p> <p> <em>BMC Biotechnology</em> 2008, <strong>8</strong><strong>:</strong>13 [Open Access]. </p> <p> </p> <h3> <strong>Abstract</strong> </h3> <h4>Background</h4> <p> Human cell lines are the most innovative choice of host cell for production of biopharmaceuticals since they allow for authentic posttranslational modification of therapeutic proteins. We present a new method for generating high and stable protein expressing cell lines based on human amniocytes without the requirement of antibiotic selection. </p> <h4>Results</h4> <p> Primary amniocytes from routine amniocentesis samples can be efficiently transformed with adenoviral functions resulting in stable human cell lines. Cotransfection of the primary human amniocytes with a plasmid expressing adenoviral E1 functions plus a second plasmid containing a gene of interest resulted in permanent cell lines expressing up to 30 pg/cell/day of a fully glycosylated and sialylated protein. Expression of the gene of interest is very stable for more than 90 passages and, importantly, was achieved in the absence of any antibiotic selection. </p> <h4>Conclusion</h4> <p> We describe an improved method for developing high protein expressing stable human cell lines. These cell lines are of non-tumor origin, they are immortalized by a function not oncogenic in human and they are from an ethically accepted and easily accessible cell source. Since the cell can be easily adapted to growth in serum-free and chemically defined medium they fulfill the requirements of biopharmaceutical production processes. </p> <p> </p> </div>
Confirmation code: Enter the code exactly as it appears. All letters are case insensitive, there is no zero.