such as "Introduction", "Conclusion"..etc
Gudrun Schiedner, Sabine Hertel, Corinna Bialek, Helmut Kewes, Gero Waschütza and Christoph Volpers
CEVEC Pharmaceuticals GmbH, Gottfried-Hagen-Str. 62, 51105 Cologne, Germany
BMC Biotechnology 2008,
8:13 [Open Access].
Human cell lines are the most innovative choice of host cell for
production of biopharmaceuticals since they allow for authentic
posttranslational modification of therapeutic proteins. We present a
new method for generating high and stable protein expressing cell lines
based on human amniocytes without the requirement of antibiotic
Primary amniocytes from routine amniocentesis samples can be
efficiently transformed with adenoviral functions resulting in stable
human cell lines. Cotransfection of the primary human amniocytes with a
plasmid expressing adenoviral E1 functions plus a second plasmid
containing a gene of interest resulted in permanent cell lines
expressing up to 30 pg/cell/day of a fully glycosylated and sialylated
protein. Expression of the gene of interest is very stable for more
than 90 passages and, importantly, was achieved in the absence of any
We describe an improved method for developing high protein
expressing stable human cell lines. These cell lines are of non-tumor
origin, they are immortalized by a function not oncogenic in human and
they are from an ethically accepted and easily accessible cell source.
Since the cell can be easily adapted to growth in serum-free and
chemically defined medium they fulfill the requirements of
biopharmaceutical production processes.
Enter the code exactly as it appears. All letters are case insensitive.