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<p> <strong><span>Direct technetium-99m labeling of anti-hepatoma monoclonal antibody <span>fragment: a radioimmunoconjugate for hepatocellular carcinoma imaging</span></span></strong> </p> <p style="line-height:9pt;"> <span>Hui Jie Bian<sup>1</sup>, Zhi Nan Chen<sup>1</sup> and Jing Lan Deng<sup>2</sup></span> </p> <p style="line-height:9pt;"> <span><strong><sup></sup></strong></span><sup><span>1</span></sup><span>Cell Engineering Research Center, Basic Medical Department, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China<br><sup>2</sup>Department of Clinical Nuclear Medicine, Xijing Hospital, Xi’an 710033, Shaanxi Province, China</span> </p> <p style="line-height:9pt;"> World J Gastroenterol <span>2000;</span><span></span><span style="font-family:Tahoma;">June</span><span style="font-family:Tahoma;"><span></span>6(3):</span><span>348-352</span> </p> <p style="line-height:9pt;"> <span><strong>Supported by National Natural Science Foundation of China, No.39700175</strong></span> </p> <p style="line-height:9pt;"> </p> <p> <strong><span>Subject headings: </span></strong><span>antibody, monoclonal; antibody fragments; </span><span>technetium-99m; hepatocellular carcinoma; liver neoplasms; radioimmunoimaging</span> </p> <p> </p> <p> <strong><span>Abstract<br></span></strong><span><strong>AIM: </strong>To directly radiolabel an anti-hepatoma mAb fragment HAb18 F(ab’)<sub>2</sub> with <sup>99m</sup>Tc by stannous-reduced method, and assess the stability, biodistribution and <em>radioimmun</em> oimaging (R</span><span>Ⅱ</span><span>).<br><br><strong>METHODS: </strong>Immunoreactive fraction was determined according to Lin dmo’s method. Ellman’s reagent was used to determine the number of thiols in the reduced F(ab’)<sub>2</sub>. Labeling efficiency and homogeneity were measured by paper chromatography, sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) and autora diography. Challenge assay involved the incubation of aliquots of labeled antibo dy in ethylenediaminetetraacetate (EDTA) and L-cysteine (L-cys) solutions with different molar ratio at 37</span><span>℃</span><span> for 1h, respectively. Investigations <em>in vivo</em> utilized nude mice bearing human hepatocellular carcinoma (HHCC) xenografts with gamma camera imaging and tissue biodistribution studies at regular intervals.<br><br><strong>RESULTS: </strong>The labeling procedure was finished within 1.5h compared with the “pretinning” method which would take at least 21h. <em>In vitro</em> studies demonstrated that the radiolabeled mAb fragment was homogen eous and retained its immunoreactivity. Challenge studies indicated that <sup>99m</sup>Tc-labeled HAb18 F(ab’)<sub>2</sub> in EDTA is more stable than in L-cys. Imaging and biodistribution showed a significant tumor uptake at 24h post-injection of <sup>99m</sup>Tc-labeled HAb18 F(ab’)<sub>2</sub>. The blood, kidney, liver and tumor uptakes at 24h were 0.56±0.09, 56.45±11.36, 1.43±0.27 and 6.57±3.01 (%ID/g)</span><span></span><span>，</span><span>respectively.<br><br><strong>CONCLUSION: </strong><sup>99m</sup>Tc-HAb18 F(ab’)<sub>2</sub> conjugate prepare d by this direct method appears to be an effective way to detect hepatoma in nude mice model.<br> </span> </p> <p style="line-height:9pt;"> </p> <p style="line-height:9pt;"> </p>
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