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<p> <strong>Summary of karyotypic analysis, and frequency of DMC</strong> </p> <p> </p> <table border="1" width="100%"><tbody><tr><td> <table border="0" cellspacing="10" cellpadding="0" width="100%"><tbody><tr><td>Cell line</td> <td>% DMC P-clone</td> <td>Insertion sites (P-clone)</td> <td>Translocation (R-pool)</td> <td>% DMC R-pool</td> <td>FISH<sup>+</sup> DMC</td> </tr><tr><td colspan="6"> <hr></td> </tr><tr><td valign="bottom">P276</td> <td valign="bottom">4</td> <td valign="bottom">der5p<sup>+</sup>[p14], 22[q11.2]</td> <td valign="bottom">t(der5p;22)(p14;q11.2)</td> <td valign="bottom">50</td> <td valign="bottom">+</td> </tr><tr><td valign="bottom">P186</td> <td valign="bottom">0</td> <td valign="bottom">3[q29], 11[p15], 13[q14]</td> <td valign="bottom">t(3;13)(q29;q14)</td> <td valign="bottom">30</td> <td valign="bottom">+</td> </tr><tr><td valign="bottom">P230</td> <td valign="bottom">0</td> <td valign="bottom">1[q41–42], t(3q;11q)[11q24]</td> <td valign="bottom">t(1;3q;11q)(q41–42;11q24)</td> <td valign="bottom">25</td> <td valign="bottom">ND</td> </tr><tr><td valign="bottom">P268</td> <td valign="bottom">0</td> <td valign="bottom">15[q24], 16[q12.1]</td> <td valign="bottom">t(15;16)(q24;q12.1)</td> <td valign="bottom">20</td> <td valign="bottom">+</td> </tr><tr><td valign="bottom">P175</td> <td valign="bottom">0</td> <td valign="bottom">6[q14–15], 10[q11.2]</td> <td valign="bottom">t(6;10)(q14–15;q11.2)</td> <td valign="bottom">19</td> <td valign="bottom">+</td> </tr><tr><td valign="bottom">P162</td> <td valign="bottom">ND</td> <td valign="bottom">3[q13], 4[q34]</td> <td valign="bottom">ND</td> <td valign="bottom">19</td> <td valign="bottom">ND</td> </tr><tr><td valign="bottom">P177</td> <td valign="bottom">ND</td> <td valign="bottom">t(3q;11q)[11q24], 15[q13]</td> <td valign="bottom">ND</td> <td valign="bottom">18</td> <td valign="bottom">ND</td> </tr><tr><td valign="bottom">P229</td> <td valign="bottom">ND</td> <td valign="bottom">ND</td> <td valign="bottom">ND</td> <td valign="bottom">13</td> <td valign="bottom">ND</td> </tr><tr><td valign="bottom">P212</td> <td valign="bottom">ND</td> <td valign="bottom">ND</td> <td valign="bottom">ND</td> <td valign="bottom">13</td> <td valign="bottom">ND</td> </tr><tr><td valign="bottom">P277</td> <td valign="bottom">ND</td> <td valign="bottom">ND</td> <td valign="bottom">ND</td> <td valign="bottom">12</td> <td valign="bottom">ND</td> </tr><tr><td valign="bottom">P280</td> <td valign="bottom">ND</td> <td valign="bottom">ND</td> <td valign="bottom">ND</td> <td valign="bottom">9</td> <td valign="bottom">ND</td> </tr><tr><td valign="bottom">P263</td> <td valign="bottom">2</td> <td valign="bottom">3[p13], 6[q26]</td> <td valign="bottom">t(3;6)(p13;q26)</td> <td valign="bottom">6</td> <td valign="bottom">–</td> </tr><tr><td valign="bottom">P27</td> <td valign="bottom">0</td> <td valign="bottom">3[p13], 16[p13.3]</td> <td valign="bottom">t(3;16)(p13;p13.3)</td> <td valign="bottom">6</td> <td valign="bottom">+</td> </tr><tr><td valign="bottom">P38</td> <td valign="bottom">0</td> <td valign="bottom">10[p11.2], 21[q22]</td> <td valign="bottom">t(10;21)(p11.2;q22)</td> <td valign="bottom">4</td> <td valign="bottom">–</td> </tr><tr><td valign="bottom">37 P-clones</td> <td valign="bottom">ND</td> <td valign="bottom">ND</td> <td valign="bottom">ND</td> <td valign="bottom">2–4</td> <td valign="bottom">ND</td> </tr><tr><td valign="bottom">P161</td> <td valign="bottom">2</td> <td valign="bottom">1[q44], 3[q24], i(14q)[q21]</td> <td valign="bottom">t(1;i14q)(q44;q21)</td> <td valign="bottom">0</td> <td valign="bottom">–</td> </tr><tr><td valign="bottom">P244</td> <td valign="bottom">0</td> <td valign="bottom">13[q22], 16[q12.1]</td> <td valign="bottom">t(13;16)(q22;q12.1)</td> <td valign="bottom">0</td> <td valign="bottom">–</td> </tr><tr><td valign="bottom">P248</td> <td valign="bottom">0</td> <td valign="bottom">3[q22], i(14q)[q32]</td> <td valign="bottom">t(3;i14q)(q22;q32)</td> <td valign="bottom">0</td> <td valign="bottom">–</td> </tr><tr><td valign="bottom">29 P-clones</td> <td valign="bottom">ND</td> <td valign="bottom">ND</td> <td valign="bottom">ND</td> <td valign="bottom">0</td> <td valign="bottom">ND</td> </tr><tr><td valign="bottom">HTD114</td> <td valign="bottom">0.5</td> <td valign="bottom">None</td> <td valign="bottom">N/A</td> <td valign="bottom">N/A</td> <td valign="bottom">ND</td> </tr></tbody></table></td> </tr></tbody></table><p> The plasmid cassette insertion sites in each P-clone were determined by FISH, using the Aprt plasmids as probes, followed by R-banding. The plasmid insertion sites are denoted by brackets and translocation breakpoints by parentheses. The identity of each translocation was determined by FISH, using WCPs as probes, and subsequently confirmed by G-banding. P27/R27 and P38/R38 were described previously [From Breger <em>et al.</em>, (9)]. The frequency of DMC was determined for each R-pool and selected P-clones and listed as percent (%). ND, not determined; N/A, not applicable. </p>
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