such as "Introduction", "Conclusion"..etc
Chloé Marchive1 *, Rim Mzid1 *, Laurent Deluc1, François Barrieu1, Julien Pirrello3, Adrien Gauthier5, Marie-France Corio-Costet2, Farid Regad3, Bernard Cailleteau4, Saïd Hamdi1 and Virginie Lauvergeat1,
1Unité Mixte de Recherche 1287, Ecophysiologie et Génomique Fonctionnelle de la Vigne, Université Bordeaux 1, Université Bordeaux 2, Institut National de la Recherche Agronomique, BP 81, F-33883 Villenave d'Ornon, France2Unité Mixte de Recherche Santé Végétale, Institut National de la Recherche Agronomique, Ecole Nationale d'Ingénieurs des Travaux Agricoles, BP 81, F-33883 Villenave d'Ornon, France3Unité Mixte de Recherche 990, Institut National de la Recherche Agronomique, Institut National Polytechnique-Ecole Nationale Supérieure Agronomique Toulouse, Génomique et Biotechnologie des Fruits, Pôle de Biotechnologie Végétale, F-31326 Castanet-Tolosan Cedex, France4ALTADIS-Institut du Tabac, Domaine de la Tour, 769 route de Sainte-Alvère, F-24100 Bergerac, France5UMR Plante–Microbe–Environnement INRA 1088, CNRS 5184, Université de Bourgogne, 17 rue Sully, BP 86510, F-21065 Dijon Cedex, France
To whom correspondence should be addressed. E-mail: [email protected]
Received 23 October 2006; Revised 17 February 2007 Accepted 5 March 2007
Abstract Pathogen attack represents a major problem for viticulture and for agriculture in general. At present, the use of phytochemicals is more and more restrictive, and therefore it is becoming essential to control disease by having a thorough knowledge of resistance mechanisms. The present work focused on the trans-regulatory proteins potentially involved in the control of the plant defence response, the WRKY proteins. A full-length cDNA, designated VvWRKY1, was isolated from a grape berry library (Vitis vinifera L. cv. Cabernet Sauvignon). It encodes a polypeptide of 151 amino acids whose structure is characteristic of group IIc WRKY proteins. VvWRKY1 gene expression in grape is regulated in a developmental manner in berries and leaves and by various signal molecules involved in defence such as salicylic acid, ethylene, and hydrogen peroxide. Biochemical analysis indicates that VvWRKY1 specifically interacts with the W-box in various nucleotidic contexts. Functional analysis of VvWRKY1 was performed by overexpression in tobacco, and transgenic plants exhibited reduced susceptibility to various fungi but not to viruses. These results are consistent with a possible role for VvWRKY1 in grapevine defence against fungal pathogens.
Key words: Fungi, plant resistance to pathogens, salicylic acid, Vitis, WRKY transcription factor
Journal of Experimental Botany 2007 58(8):1999-2010. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/).
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