such as "Introduction", "Conclusion"..etc
Bioconversion of (+)- and (-)-alpha-pinene to (+)- and (-)-verbenone by plant cell cultures of Psychotria brachyceras and Rauvolfia sellowii
Renata Pereira LimbergerPrograma de Pós-Graduação em Ciências FarmacêuticasFaculdade de FarmáciaUniversidade Federal do Rio Grande do SulAv. Ipiranga 2752, 90.610.000Porto Alegre, RS, BrazilTel: 55 51 33085297Fax: 55 51 33085437E-mail: email@example.com
Adriana Mendes AleixoFaculdade de Engenharia, Arquitetura e UrbanismoUniversidade Metodista de PiracicabaRodovia Santa Bárbara Iracemápolis Km 1, 13450-000Santa Barbara D'oeste, SP, BrazilTel: 19 31241782E-mail: firstname.lastname@example.org
Arthur Germano Fett-NetoPrograma de Pós-Graduação em Biologia Celular e MolecularCentro de BiotecnologiaUniversidade Federal do Rio Grande do SulCP 15005 Agronomia, 91509-900Porto Alegre, RS - BrazilTel: 51 33087642Fax: 51 33087309E-mail: email@example.com
Amélia T. Henriques*Programa de Pós-Graduação em Ciências FarmacêuticasFaculdade de FarmáciaUniversidade Federal do Rio Grande do SulAv. Ipiranga 2752, 90.610.000Porto Alegre, RS, BrazilTel: 55 51 33085417Fax: 55 51 33085437E-mail: firstname.lastname@example.org
Financial support: CNPq, CAPES and FAPERGS.
Keywords: alpha-pinene, biotransformation, verbenol, verbenone.
2,4-D: 2,4-dichlorophenoxyacetic acidEtOAc: ethyl acetateGC: gas chromatographyGC-MS: gas chromatography-mass spectrometryMS: Murashige and SkoogNMR: Nuclear Magnetic Resonance Spectroscopy
This work describes the bioconversion of (-)- and (+)-alpha-pinene (2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene), targeted at the production of (-)- and (+)-verbenone (4,6,6-trimethyl-bicyclo (3.1.1) hept-3-en-2-one), respectively, using Psychotria brachyceras and Rauvolfia sellowii cell suspension cultures. P. brachyceras showed selectivity to (-)-alpha-pinene with 80.9% conversion (relative integrated area gas chromatography-mass spectrometry (GC-MS)) of (-)-verbenone in 10-day-incubation, whereas R. sellowii was able to convert both pinene enantiomers (37.6% conversion of (-)-verbenone in 7-day-incubation and 32.2% conversion of (+)-verbenone in 10-day-incubation). In both systems trans-verbenol was formed as main product and then slowly biocatalyzed to verbenone. Verbenone were also present among the autoxidation products during control experiments, but in much lower amounts and accompanied by several by-products, highlighting the usefulness of the biotransformation process.
Electronic Journal of Biotechnology, Vol. 10 No. 4, Issue of October 15, 2007.
Enter the code exactly as it appears. All letters are case insensitive, there is no zero.