Scratching the (T cell) surface
Joerg Ermann, Chan D Chung and C Garrison Fathman
Division of Immunology and Rheumatology, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
Using a genome-scale approach to study transcription levels in a human CD8+ T-cell clone, a recent study has suggested that the repertoire of molecules on the surface of T cells is close to being completely characterized.
Source: Genome Biology 2003, 5:202
Over the last few years technological advances have made it possible to study, in parallel, the expression of thousands of genes in cells, tissues or organisms. While this genome-scale approach to gene-expression analysis has been touted by some as the new 'golden era' of hypothesis-unlimited discovery-driven research , it is apparent that our ability to make sense of the vast accumulations of data does not always keep up with our ability to generate them. Many groups have used technologies such as microarrays or serial analysis of gene expression (SAGE) to analyze gene expression in cells of the immune system. A recent article by Evans et al. in Immunity  attempting to expand beyond the simple description of differential gene-expression patterns arrives at a bold conclusion. The article's title is intriguing - The T-cell surface - how well do we know it? - and the conclusion is that we know it quite well.
Not surprisingly, molecules on the surface of T cells are of great interest to immunologists. Much information has been generated about T-cell-specific surface molecules and their function since the first monoclonal antibodies against leukocyte surface markers were made in the late 1970s. Attempts to compare the specificities of different monoclonal antibodies and to identify their targets led to the development of the cluster of differentiation (CD) nomenclature . Most of the CD antigens turned out to be proteins (a few CD antibodies detect carbohydrate modifications) and the respective genes have been cloned in both humans and mice. Over time the CD system thus transformed into a classification for leukocyte surface molecules, rather than antibodies. Currently, there are 247 assigned CDs . Almost 200 additional molecules are being considered for CD status during the current Eighth International Workshop on Human Leukocyte Differentiation Antigens that will culminate in the HLDA8 Conference in Adelaide, Australia in December 2004 .
The molecules on the surface of T cells belong to very diverse structural and functional classes, and include components of the immunoreceptors on T, B and NK cells, adhesion molecules, and cytokine and chemokine receptors. Some CDs have proven to be useful markers of subpopulations of cells with strikingly different functions. Most T cells express CD3 and an αβ T-cell receptor (TCR) paired with either the CD4 or the CD8 molecule as co-receptor. CD4+ αβ T cells recognize antigens presented by antigen-presenting cells in the context of major histocompatibility complex (MHC) class II molecules. Their main effector function is the production of cytokines and the facilitation of immune responses. A subclass of CD4+ T cells that has recently gained attention comprises regulatory/suppressor T cells, which are characterized by the constitutive expression of CD25 - the α-chain of the interleukin 2 (IL-2) receptor. These cells are thought to negatively regulate immune responses and to prevent uncontrolled autoimmunity. CD8+ αβ T cells recognize antigens in the context of MHC class I molecules, which are expressed on most somatic cells. Upon activation, CD8+ T cells develop into cytolytic T lymphocytes (CTLs) ready to kill cells infected by intracellular pathogens, such as viruses, or eradicate tumors cells.
In their article on the T-cell surface, Evans et al.  used SAGE to study gene expression in a human CD8+ CTL clone. The SAGE library that was generated (referred to as the CTL library) contained 71,174 SAGE tags representing 20,204 distinct sequences. This number was estimated to cover all of the transcripts whose expression level was at or above 0.008% of the transcriptome (that is having at least 22 copies per cell). The library included 111 genes with, or being considered for, CD status. Several pairwise comparisons with unrelated SAGE libraries were then performed.
The central analysis of the paper starts with a comparison of the CTL library with a library derived from cerebellum; 1,098 transcripts were significantly more abundant in the CTL library. Among these, about a quarter of the transcripts with known function coded for proteins involved in protein/mRNA synthesis, a result that was thought to reflect the proliferating versus non-proliferating character of the two cell/tissue types (CTL versus cerebellum). Interestingly, the set of genes that was highly differentially expressed in the CTLs was enriched for surface markers, signaling molecules and soluble mediators. In an attempt to find a core set of CTL-specific genes, additional comparisons were performed between the CTL library and SAGE libraries from ovary epithelium (as a type of proliferating cell) and a panel of tumor libraries. This resulted in a shortened list of 387 CTL-specific transcripts.
Notably, at all stages of comparison 42-45% of the transcripts lacked an assigned function. Of the known genes in the final list of 387 specific transcripts in the CTL library, 27% were cell-surface molecules, including TCR components, CD2, CD5, and CD8. Evans et al.  then asked how many of the unknown CTL-specific transcripts encoded surface molecules. Sequences representing UniGene clusters  were analyzed for signatures of surface molecules by domain analysis, looking for transmembrane regions or other domains characteristic of leukocyte surface molecules, and by BLAST searches for related genes with known function. Surprisingly, only 2 of the 97 (2%) UniGene clusters analyzed showed some potential for encoding novel surface molecules. The authors therefore concluded that "the cell-type-specific composition of the resting CD8+ T-cell surface is now largely defined."
How complete and cell-type-specific is this list of 387 genes? The CTL SAGE library of 20,204 transcripts represents the transcriptome of the CTL clone. A detailed discussion of the limitations of the SAGE method is beyond the scope of this article; suffice it to say that it is possible that functionally important genes may be missing from the library due to low mRNA expression levels, chance or because they lack the target sequence for the tagging enzyme used in the SAGE protocol . In order to get to the final set of 378 CTL-specific genes Evans et al.  eliminated all transcripts that were present at comparable levels in unrelated libraries. This powerful approach seems to have validated itself by the fact that TCR components and other principal T-cell markers were present in the shortlist of 387 genes. The method used is not unbiased, however. The choice of libraries (in this case cerebellum, ovary epithelium, and a panel of tumor cell lines that were not specified further), data quality, and the algorithms used for comparison can be expected to have an enormous impact on the results obtained. It is easy to see how functionally important genes might get lost because they are expressed at sufficiently high levels in one of the cell or tissue types used for comparison. A list of cell-type-specific genes derived by this method of successive in silico subtraction defines a cell-type-specific gene-expression pattern against the transcriptional background of the cell or tissue types used for comparison. It is not a list of all genes relevant for cell-type-specific function.
The major finding of the Evans et al. study  is that among the 387 CTL-specific transcripts 27% of the known genes encoded cell-surface molecules, whereas only 2% of the unknown genes showed some potential in that regard. The implication is that the catalog of CTL surface molecules is close to being complete. While it is not unreasonable to assume that the concerted efforts over the last two decades to characterize surface molecules on leukocytes have led to a situation where most CTL-specific surface molecules are known , some questions remain. Is this finding unique for CTLs (or for leukocytes in general)? What would be the result of a similar analysis in, for instance, ovary epithelium? Were there unknown cell-surface molecules in the CTL SAGE library? If so, at what point of the stepwise subtraction process did these transcripts get eliminated? It has been noted that leukocytes share many surface molecules with neuronal cells and epithelial cells , the very cells used for subtraction by Evans et al. . An alternative experimental approach to analyzing the incidence of unknown cell-surface molecules might be to generate SAGE libraries from microsomal and free-ribosomal mRNA pools generated through equilibrium density centrifugation. This approach has been demonstrated to discriminate secretory and cell-surface molecules from nonsecretory proteins quite efficiently [9,10]. One would expect a significantly lower percentage of unknown transcripts in the secretory/surface molecule fraction.
Only the CTL SAGE library was actually generated by Evans et al. . The other libraries used for comparison were derived from publicly available databases. Open access to primary gene-expression data is essential, not only for enabling researchers to reproduce published analyses, but also to allow for novel experimental approaches that incorporate relevant data generated by others. Important information can be gained by comparing genome-wide expression data across large numbers of samples. In a recent, extreme example, 3,283 DNA microarrays from human, Drosophila, Caenorhabditis elegans and yeast were used to define evolutionarily conserved genetic modules of co-expressed genes . SAGE data have been publicly available on SAGEMap [12,13] for a number of years. Microarray data are far more complex, but a standard for the annotation of microarray data (Minimal Information About a Microarray Experiment; MIAME)  and a platform-independent data exchange format (Microarray Gene Expression Markup Language; MAGE-ML)  have been developed. Furthermore, public repositories for microarray data such as ArrayExpress [16,17] and GeoBus [18,19] are now available.
SAGE has the advantage over current microarray technology of measuring absolute transcript abundance. Nevertheless, there are some limitations as to what can be said about the T-cell surface by studying mRNA levels. First, for a number of surface molecules, such as CD45, a variety of functionally important splice variants have been described  that cannot be distinguished by the 3' SAGE tag. Second, mRNA levels correlate poorly with protein abundance . Third, posttranslational protein modifications can be functionally relevant; for example, glycosylation of CD8 has been demonstrated to affect thymocyte selection by influencing activation thresholds . Fourth, T-cell activation involves re-localization of surface molecules leading to the formation of the immunological synapse, a supramolecular cluster at the contact zone between antigen-presenting cell and the T cell . These early events precede changes in gene expression.
Finally, it seems important to note that the T-cell surface is an abstraction. T cells comprise quite different subsets of cells at variable activation states. As pointed out by Evans et al. , the finding that most of the molecules on the T-cell surface appear to be known applies strictly only to a resting CD8+ T-cell clone in vitro. 'The T-cell surface - how well do we know it?' is an important question on our way into the post-genomic era of immunology. But even with complete lists of the genes expressed in certain T-cell subpopulations, much more needs to be learned about the regulation and complex interactions of the proteins they encode. We are just scratching the (T cell) surface.
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