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Methods & Techniques

Biology laboratory techniques include articles that describe different methods or procedures applied in biological research.

Methods & Techniques Articles

Mouse keratinocyte cultures
Isolation of epidermal keratinocytes from neonatal mice

Date: 21 Aug 2007, Rating: not rated

TCA Precipitation
TCA Precipitiation of proteins

Date: 21 Aug 2007, Rating: 3.00

Thawing Cells
Standard protocol for thawing of cells. Make sure it is suitable for your specific cell-type.

Date: 21 Aug 2007, Rating: 1.50

PAPS (phosphoadenosine phosphosulfate) preparation
This protocol is used to prepare PAPS, a sulfate donor used in for example assays measuring the activity of sulfotransferases.

Date: 21 Aug 2007, Rating: not rated

Children's surgeon to present results of new anti-rejection protocol at World Transplant Congress
Children's Hospital of Pittsburgh Surgeon and Researcher Rakesh Sindhi, MD, to discuss new protocol that has shown to improve patient outcomes

Date: 21 Aug 2007, Rating: not rated

Preparing for an influenza pandemic: A triage protocol in the face of limited resources
The recent outbreaks of avian influenza (H5N1) around the world have placed a renewed emphasis on preparing for an influenza pandemic in humans

Date: 21 Aug 2007, Rating: not rated

Ottawa aggressive protocol for acute atrial fibrillation is successful
There is no consensus on Emergency Department management of acute atrial fibrillation (AAF) or atrial flutter (AAFL).

Date: 21 Aug 2007, Rating: not rated

A frenzy of fruit fly methods featured in Cold Spring Harbor Protocols
For the past century, fruit flies—or Drosophila—have provided innumerable insights into the genetics and biology of development, learning and memory, behavior, vision, and other processes.

Date: 21 Aug 2007, Rating: not rated

Isolation of murine splenocytes
In order to study spleen cells (e.g. lymphocytes, granulocytes, other immune cells), it helps to make single-cell suspensions so that the cells can be manipulated ex vivo easily.

Date: 21 Aug 2007, Rating: 3.33

Intracellular cytokine staining for flow cytometry (mouse)
Staining for intracellular cytokines, followed by flow cytometry analysis, can provide single-cell information about a cell population that one cannot obtain from surface staining or ELISA

Date: 21 Aug 2007, Rating: not rated

Rice Researchers Develop First Fully Automatic Method To Track 3-D Movement Of Cancer Cells
Cancer researchers know the disease spreads from organ to organ in a non random pattern

Date: 6 Feb 2007, Rating: 4.81

Toward Pinpointing The Location Of Bacterial Infections
In an advance in the emerging field of bacterial imaging, scientists are reporting development of a method for identifying specific sites of localized bacterial infections in living animals.

Date: 4 Jan 2007, Rating: 1.00

Adenine 'Tails' Make Tailored Anchors For DNA
Researchers have demonstrated a deceptively simple technique for chemically bonding single strands of DNA to gold.

Date: 3 Jan 2007, Rating: 1.33

New Blood Test Could Detect Lung Cancer In Its Earliest Stages
Lung cancer is often deadly by the time doctors have detected it, but scientists at Duke University Medical Center are developing a non-invasive test that could detect lung cancer in its earliest stages, while it is still treatable.

Date: 1 Feb 2007, Rating: 1.43

Scientists Make First Step Towards 'Holy Grail' Of Crystallography
Scientists from Imperial College London and the University of Surrey have developed a new technique for crystallising proteins, a discovery which could help speed up the development of new medicines and treatments.

Date: 29 Jan 2007, Rating: 9.00

Optical Bioimaging: From Living Tissue to a Single Molecule: Single-Molecule Visualization of Cell Signaling Processes of Epidermal Growth Factor Receptor
Single-molecule analysis has proven to be a powerful technology to analyze intracellular protein systems

Date: 29 Jan 2007, Rating: 3.67, 9 pages

Importance of quantifying insulin secretion in relation to insulin sensitivity to accurately assess beta cell function in clinical studies
Insulin sensitivity and insulin secretion are mutually related such that insulin resistance is compensated by increased insulin secretion.

Date: 29 Jan 2007, Rating: 4.53, 11 pages

Mass Spectrometry as a Diagnostic and a Cancer Biomarker Discovery Tool
In this review, the author concentrated more on questions that cast doubt on the results reported and propose experiments to investigate these questions in detail, before the technique is used at the clinic.

Date: 29 Jan 2007, Rating: 4.00, 17 pages

Imaging of chlorophyll a fluorescence: theoretical and practical aspects of an emerging technique for the monitoring of photosynthetic performance
This paper discusses some of the theoretical and practical issues associated with the imaging of Chl a fluorescence and with Chl a fluorometry in general.

Date: 29 Jan 2007, Rating: 1.75, 9 pages

Metabolic Mapping of Proteinase Activity with Emphasis on In Situ Zymography of Gelatinases : Review and Protocols
In situ zymography provides data that extend our understanding of the role of specific proteinases in various physiological and pathological conditions.

Date: 23 Nov 2006, Rating: 5.06, 10 pages