Zymogen

... process is much more complex mechanically, but that doesn't need to brought into the discussion. Pepsin is the active form of a protein called a zymogen which is enzyme that has to be cleaved to be activated. Pepsin as well as other enzymes (enzymes are all proteins but not all proteins are enzymes) ...

See entire post

... either 57 or 45 Kda. By auto proteolytic cleavage the enzyme is activated during the incubation with developing buffer. Refered wikipedia "A zymogen (or proenzyme) is an inactive enzyme precursor. A zymogen requires a biochemical change (such as a hydrolysis reaction revealing the active ...

See entire post

... stained for protein. Light bands against a dark background show up in regions of the gel where there was enzyme activity during the incubation. A zymogen is simply the "inactive" precursor of an enzyme, or the proenzyme; for example, chymostrypsinogen is the proenzyme of chymotrypsin; ...

See entire post

... for A) in the answer is 340 and 200 kDa bands? This seems way too simple of an answer. I checked over my course text book and there is nothing on zymogen in there. If someone could point me in the right direction it would be greatly appreciated, thanks for your time!

See entire post

... for A) in the answer is 340 and 200 kDa bands? This seems way too simple of an answer. I checked over my course text book and there is nothing on zymogen in there. If someone could point me in the right direction it would be greatly appreciated, thanks for your time!

See entire post