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Zwitterion - definition from
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Re: Finding the net charge of amino acid

... three pKa's and the denomimator would be "3" rather than "2" and so on. The only ionic species present when the pH = pI is the zwitterion where both the amino group and the carboxylate are ionzed--the amino group is +1 and the carboxylate is -1. As you move above the pI, the ...

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by blcr11
Tue Sep 30, 2008 12:07 pm
Forum: Molecular Biology
Topic: Finding the net charge of amino acid
Replies: 19
Views: 58814

Glycine in Western Blot buffers

... you have loaded your samples and applied a current, your proteins, the chloride ions, and the glycine start to flow throw the gel. Glycine is a zwitterion in the stacking portion of the gel (~pH 7). The chloride ions take the lead and the glycine in the running buffer takes up the rear: your ...

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by snowcapk
Tue Aug 12, 2008 4:00 am
Forum: Molecular Biology
Topic: Glycine in Western Blot buffers
Replies: 2
Views: 15698

biochemistry for dummies (me, that is...)

Note that it contains both an alkaline group and an acidic group. The term you're looking for is probably zwitterion.

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by mith
Sun Apr 27, 2008 2:47 pm
Forum: Molecular Biology
Topic: biochemistry for dummies (me, that is...)
Replies: 8
Views: 12859

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