zone centrifugation --> density gradient centrifugation
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... IPTG 2 pSP1 Kan 3 Red Colony Amp IPTG 4 Red Colony Amp 5 Large Clearing Zone Amp IPTG 6 Large Clearing Zone Amp 7 Small Clearing Zone Amp IPTG 8 ... and leave at room temperature for 5 min.  Pellet cell debris by centrifugation in microfuge for 10 min and full speed. Retain the supernatant ...
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