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Zebrafish

zebrafish

(Science: cell biology, genetics) brachydanio rerio, species of small fresshwater aquarium fish with a transparent embryo making it possible to follow progeny of single cells until quite late stages of development. This, together with the availability of mutant lines make it an important preparation for the study of vertebrate cell lineage and developmental biology.


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microRNA in situ hybridization protocol: zebrafish

... did some fine work on this one. Lagendijk AK, Moulton JD, Bakkers J. Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues. Biology Open. 2012;[Advance Online Publication] doi:10.1242/bio.2012810 http://bio.biologists.org/content/early/2012/04/11/bio.2012810.full

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by jonmoulton
Fri Apr 27, 2012 4:32 pm
 
Forum: Molecular Biology
Topic: microRNA in situ hybridization protocol: zebrafish
Replies: 0
Views: 1009

Queston about Zebrafish Whole mount Immunohistochemistry?

Hello, Has anyone done an IHC experiment in Zebrafish embryos for detecting 8-oxoG? I was wondering what would be the best fixative to use? Thanks

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by Vivkrish
Tue Apr 24, 2012 7:58 pm
 
Forum: Molecular Biology
Topic: Queston about Zebrafish Whole mount Immunohistochemistry?
Replies: 0
Views: 937

Video: electroporation in zebrafish tails

Anyone for a video? Hyde DR, Godwin AR, Thummel R. In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin. J Vis Exp. 2012;(61):e3632 DOI: 10.3791/3632 http://www.jove.com/video/3632/in-vivo-electroporation-of-morpholinos-into-the-regenerating-adult-zebrafish-tail-fin

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by jonmoulton
Thu Mar 29, 2012 8:00 pm
 
Forum: Molecular Biology
Topic: Video: electroporation in zebrafish tails
Replies: 0
Views: 886

gene-break transposons

This is gene engineering at the edge: "implementing gene-break transposons to create expression-tagged, revertible mutations in zebrafish genes". http://zfishbook.org/ Here's a description of the technology: Clark KJ, Balciunas D, Pogoda HM, Ding Y, Westcot SE, Bedell VM, ...

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by jonmoulton
Wed Jan 11, 2012 11:02 pm
 
Forum: Genetics
Topic: gene-break transposons
Replies: 0
Views: 1135

promoter location

Greetings, I am going to clone the promoter of a zebrafish gene. Do you have any idea that how I should find its location? Thank you :) Sahar

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by sahart
Tue Jan 18, 2011 4:12 am
 
Forum: Molecular Biology
Topic: promoter location
Replies: 5
Views: 2665
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