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Yrk kinase

Yrk kinase

(Science: enzyme) Non-receptor type protein kinase of src family; consists of 536 amino acids and has all the canonical features of a src kinase; expressed at elevated levels in neuronal and haematopoietic tissues; amino acid sequence given in first source

Registry number: EC 2.7.1.-

Synonym: yes-related kinase, yrk protein

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cdc25 and tyrosin-15

... cdc25 gene of the fission yeast Saccharomyces pombe encodes a phosphatase which dephosphorylates the tyrosine 15 residue of the cyclin-dependent kinase (Cdk). Dephosphorylation of tyrosine-15 of Cdk activates its kinase activity. You introduced a 2nd copy of the cdc25 gene under control of the ...

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by jb0378
Wed Jul 29, 2009 10:18 pm
Forum: Cell Biology
Topic: cdc25 and tyrosin-15
Replies: 1
Views: 97

Ras Pathway

... to show how the cell cycle in the budding yeast, Saccharomyces cerevisiae, is temporarily halted during mating. Be sure to specify the role of MAP kinase pathway(s) in this process. The following questions focus on experimental observations that are related to determining the sequence in which ...

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by biologybuzz
Mon Apr 27, 2009 10:36 pm
Forum: Cell Biology
Topic: Ras Pathway
Replies: 0
Views: 398

Single PCR tube DNA Extraction Method

... here are my ideas and method and please comment and give suggestions. PCR Tube- Mixed inside PCR tube 10ul of 1% SDS solution 10ul of Protein K Kinase 15ul of Nucleas free water. 15 Beads from a Zippy Fecal Extraction kit, Then Vortexted at the highestl level for 30 minutes. PCR tube then Placed ...

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by Swordy
Mon Apr 27, 2009 5:53 pm
Forum: Molecular Biology
Topic: Single PCR tube DNA Extraction Method
Replies: 0
Views: 234

Creating Cell Lines

Is the receptor in GPCR/Kinase/any other enzyme family? If you know the receptor, you should try to knock it out. You need a stable cell line though. That is a big project. But many times, you get multiple receptors for one ligand. siRNA ...

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by LifeTein
Sat Apr 25, 2009 2:21 pm
Forum: Cell Biology
Topic: Creating Cell Lines
Replies: 3
Views: 374

Re: Frog Egg Physilogy

... DNA kit, A Zippy Fecal kit and many others. I have also tryed some homegrown methods involveing in different combinations Bead Bashing, Protein Kinase, SDS, HCl, NaOH, Tris, Freezing, and Boiling. None of these have worked in the slightest except to get mtDNA which as far as i know is useless ...

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by Swordy
Thu Apr 23, 2009 5:51 pm
Forum: Zoology Discussion
Topic: Frog Egg Physilogy
Replies: 2
Views: 472
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