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Yeast vectors

(noun) Plural form of yeast vector.

See yeast vector for definition and additional information.

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galactose metabolism

plz help....i need this urgently......i have isolated a mutant cell of yeast that cannot take part in a particular step of galactose metabolism...in ... gene responsible for it.....i have transformed the cell with plasmid vectors containing fragments of genomes of wild type yeasts.....after growing ...

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by APmicro
Thu Oct 11, 2012 3:46 am
Forum: Cell Biology
Topic: galactose metabolism
Replies: 0
Views: 1053

How to replace gene with modified gene [unequal length] ?

... complicated. From what I remember from introducing pGAPZ and pPICZ vectors into Pichia, they just contain the promotor, which is usually in the yeast genome and the vector is cut in the promotor to be linearized and then simply inserted ...

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by JackBean
Wed Mar 21, 2012 11:23 am
Forum: Genetics
Topic: How to replace gene with modified gene [unequal length] ?
Replies: 3
Views: 1919

Expression Vector vs Cloning Vector

... expressing in bacteria or have vector working both in bacteria and yeast (like pPICZ and pGAPZ). However, the point of using two vectors is such, that you can use your protein for plenty of applications (e.g. expression, ...

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by JackBean
Sat Sep 25, 2010 2:52 pm
Forum: Molecular Biology
Topic: Expression Vector vs Cloning Vector
Replies: 1
Views: 2954

Multiple genes cloning

... antiviral proteins may be transfected to bacteria, the antibacterial to yeast. You should prepare the appropriate expression vectors, pay attention to the size, promoter, selection marker. Treat the cells accordingly ...

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by GreenDog
Thu Jan 15, 2009 9:33 am
Forum: Molecular Biology
Topic: Multiple genes cloning
Replies: 7
Views: 5675

Some MCQs

... to a recessive genetic disease allele which statement is TRUE: BAC vectors in e.coli are based on the pMBI replicon and replicon and replicate ... vectors becasue there are no natural plasmids in mammalian cells yeast vectors based on the 2-micron plasmid replicate at copy numbers of ...

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by biology_06er
Tue Nov 13, 2007 2:25 am
Forum: General Discussion
Topic: Some MCQs
Replies: 3
Views: 5307
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