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Yeast vector

Definition

noun, plural: yeast vectors

A yeast cell that functions as a vector, cloning specific DNA fragments.


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Choosing a yeast vector

... several plant arsenic transporters and would like to express them in yeast to see if they can partially rescue an arsenic-sensitive yeast strain. I am wondering how I choose an expression vector that is compatible with my yeast strains. Have done a lot of internet searches ...

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by alyson2324
Thu Aug 02, 2012 4:54 pm
 
Forum: Molecular Biology
Topic: Choosing a yeast vector
Replies: 0
Views: 1444

A question I'm finding hard to answer (recombinant protein)

... otehr hand, is not of big concern, because it's usually part of your vector, which is specifically constructed for desired host, thus the promotor ... Just to make it clear, the host does not have to be bacteria, it can be yeast, cell culture (either plant, insect or even human) or whole eukaryotic ...

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by JackBean
Wed May 02, 2012 7:11 am
 
Forum: Cell Biology
Topic: A question I'm finding hard to answer (recombinant protein)
Replies: 9
Views: 6766

How to replace gene with modified gene [unequal length] ?

... complicated. From what I remember from introducing pGAPZ and pPICZ vectors into Pichia, they just contain the promotor, which is usually in the yeast genome and the vector is cut in the promotor to be linearized and then simply inserted ...

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by JackBean
Wed Mar 21, 2012 11:23 am
 
Forum: Genetics
Topic: How to replace gene with modified gene [unequal length] ?
Replies: 3
Views: 1878

yeast expression vector

Hi All, I’m looking for a vector for expression of a third bridging protein in the Invitrogen Proquest yeast two-hybrid system. Does anyone have or know of a yeast expression vector that suits the following requirements: ...

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by fcs3
Thu Dec 08, 2011 11:12 pm
 
Forum: Molecular Biology
Topic: yeast expression vector
Replies: 1
Views: 999

Expression Vector vs Cloning Vector

... you even do, like e.g. if you're expressing in bacteria or have vector working both in bacteria and yeast (like pPICZ and pGAPZ). However, the point of using two vectors is such, that you can ...

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by JackBean
Sat Sep 25, 2010 2:52 pm
 
Forum: Molecular Biology
Topic: Expression Vector vs Cloning Vector
Replies: 1
Views: 2926
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