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Yac

YAC --> yeast artificial chromosome

(Science: molecular biology) A vector system that allows extremely large segments of dNA to be cloned.

Useful in chromosome mapping, contiguous yeast artificial chromosomes covering the whole drosophila genome and certain human chromosomes are available.

Acronym: YAC


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Sequencing Primers

... specified sequences are located in an unknow sequence. Restriction enzymes are also used to insert a piece of DNA into a plasmid, cosmid, BAC and YAC for cloning. Plasmids, etc., all have the advantage of containing restriction sites where the plasmid can be cut and an unknown piece of DNA is ...

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by DougB
Mon Apr 16, 2012 8:25 pm
 
Forum: Genetics
Topic: Sequencing Primers
Replies: 4
Views: 3102

Sequencing Primers

Clone in a plasmid/cosmid/BAC/YAC Take a primer just beside cloning site (or better at both end) Sequence Design a primer at the end of your sequence(s) Sequence Repeat until you get the whole sequence, whether you go in 1 or 2 directions ...

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by canalon
Sat Mar 31, 2012 2:16 am
 
Forum: Genetics
Topic: Sequencing Primers
Replies: 4
Views: 3102

plasmids

... for example the one below Ori of the plasmid is the only responsible element for duplication. One has Oris of virii, bacteria and yeasts (YAC). Copy number maybe regulated in numerous ways.

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by siroma
Thu Jun 28, 2007 10:14 pm
 
Forum: Cell Biology
Topic: plasmids
Replies: 13
Views: 6545

what is the full length transcipts ?

... all. And if u mean on genetic libraries well it's not so hard to constate size of gene so than you'll now which vector to use(plasmid, virus, BAC,YAC,....)

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by zami'87.
Mon Jun 06, 2005 1:15 pm
 
Forum: Molecular Biology
Topic: what is the full length transcipts ?
Replies: 5
Views: 3962


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