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Xylene cyanol ff

xylene cyanol FF

An acidic triphenylmethane dye used for histochemical staining of haemoglobin peroxidase and as a tracking dye for dNA sequencing in electrophoresis.


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SYBR gold in acrylamide gel

... for the visualization of Sybr gold fluorescence. Since you see the xylene cyanol, that means that the DNA is correctly loaded. Do you put a molecular weight ladder. ...

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by canalon
Sat Nov 01, 2008 3:12 am
 
Forum: Molecular Biology
Topic: SYBR gold in acrylamide gel
Replies: 4
Views: 5451

Re: SYBR gold in acrylamide gel

... filtre? I think that the DNA doesn't go out of the gel because the xylene cyanol was in the run and it run more or less at 105bp on 6% acrylamide gel. May be I should ...

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by Kiraya
Fri Oct 31, 2008 9:15 am
 
Forum: Molecular Biology
Topic: SYBR gold in acrylamide gel
Replies: 4
Views: 5451

loading buffer

... sds? what is the difference with a loading buffer that contains just xylene cyanol FF,bromophenol blue, glycerol, EDTA,ddH2O? if someone knows exactly the concetrations ...

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by hara
Wed Jan 17, 2007 1:56 pm
 
Forum: Molecular Biology
Topic: loading buffer
Replies: 2
Views: 1333


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