Xylene

... for several days. Put the fixed fruit into serie of organic solvent concentration gradient- 30% ethanol, 50% ethanol, 70% ethanol, 96% ethanol, xylene or acetone...evaporation. Keep the fruit for several hours in each solution. You will se what you get. Or alternatively, once water is changed ...

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... (1) Is the dehydration step very important for cell capture? If yes, how can I perform this step? (the literatures refers the graded ethanols and xylene, but ethanol could quench my GPF signal) (2) What kind of slide is better for single cell capture by IR laser? Glass or membrane? And the thickness ...

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... Red blood cells do not look red under a microscope without a stain. I would suggest doing the same stain.. very easy, all you need is some xylene, ethanol and H and E.. Once you have stained your sample, WBC are bigger and nucleated. The picture shows a lymphocyte and what looks like a ...

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... The protocols vary widely, but the idea of different concentrations is to remove water from the sample (or add water and remove ethanol/methanol/xylene etc if you want to rehydrate and do all this in reverse order). If you used 70% ethanol straight away, it gets diluted too much and there's still ...

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... system are equiped for EtBr and the dark red filter is far from optimal for the visualization of Sybr gold fluorescence. Since you see the xylene cyanol, that means that the DNA is correctly loaded. Do you put a molecular weight ladder. If you see it then the problem is likely your sample. ...

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Xylene

Results from our forum

Microorganisms and organic preservation?

question about LCM

Re: How's red blood cells look like under the microscope?

using ethanol 50% then 70%??

SYBR gold in acrylamide gel

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Xylene

Results from our forum

Microorganisms and organic preservation?

question about LCM

Re: How's red blood cells look like under the microscope?

using ethanol 50% then 70%??

SYBR gold in acrylamide gel