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(Science: chemistry) Any of a group of three metameric hydrocarbons of the aromatic series, found in coal and wood tar, and so named because found in crude wood spirit. They are colourless, oily, inflammable liquids, C6H4.(CH3)2, being dimethyl benzenes, and are called respectively orthoxylene, metaxylene, and paraxylene.

Synonym: xylol.

Each of these xylenes is the nucleus and prototype of a distinct series of compounds.

Origin: Gr. Xylon wood.

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Methylene Blue Solution use in staining variety of bacteria?

... (10 dips, sections turn green), 2 changes of 100% alcohol (10 dips each) (alcohol used for dehydration removes some of the stain). 5. Clear in xylene or xylene substitute. 6. Mount with resinous mounting medium.

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by scytek
Sun Feb 22, 2015 9:22 am
Forum: General Discussion
Topic: Methylene Blue Solution use in staining variety of bacteria?
Replies: 0
Views: 272

Problems with FluoroJade staining

... everything away from light as possible) 30 minutes in FluoroJade solution 0.0004% 7) rinse in 3x1minute of dH20 8) dry overnight 9) one minute in xylene, DPX and dry My questions are: A) Should I rehydrate the slides slower than in the protocol (but why would anyone succeed then?) doing ethanol ...

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by fabulousparrot
Mon May 20, 2013 1:47 pm
Forum: Molecular Biology
Topic: Problems with FluoroJade staining
Replies: 0
Views: 2117

Microorganisms and organic preservation?

... for several days. Put the fixed fruit into serie of organic solvent concentration gradient- 30% ethanol, 50% ethanol, 70% ethanol, 96% ethanol, xylene or acetone...evaporation. Keep the fruit for several hours in each solution. You will se what you get. Or alternatively, once water is changed ...

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by triton
Wed Aug 22, 2012 7:01 pm
Forum: Microbiology
Topic: Microorganisms and organic preservation?
Replies: 1
Views: 4562

question about LCM

... (1) Is the dehydration step very important for cell capture? If yes, how can I perform this step? (the literatures refers the graded ethanols and xylene, but ethanol could quench my GPF signal) (2) What kind of slide is better for single cell capture by IR laser? Glass or membrane? And the thickness ...

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by johnmay
Mon Oct 17, 2011 4:43 pm
Forum: Molecular Biology
Topic: question about LCM
Replies: 0
Views: 1565

Re: How's red blood cells look like under the microscope?

... Red blood cells do not look red under a microscope without a stain. I would suggest doing the same stain.. very easy, all you need is some xylene, ethanol and H and E.. Once you have stained your sample, WBC are bigger and nucleated. The picture shows a lymphocyte and what looks like a ...

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by goonwolf420
Thu May 20, 2010 7:59 am
Forum: Cell Biology
Topic: How's red blood cells look like under the microscope?
Replies: 6
Views: 123003
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