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Western blot analysis

Western blot analysis

A procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pre-or post-tagged with a labelled secondary protein.

See: immunoblot.

Synonym: western blot, western blotting.

Origin: coined to distinguish it from eponymic Southern blot a.

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Scholarly article help

Figure 2C: What is a western blot? What is it supposed to show? They are quite clear what is in what lane in the legend: Western blot analysis showing the presence and absence of CdhA expression in ...

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Thu Mar 10, 2011 8:16 pm
Forum: Microbiology
Topic: Scholarly article help
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protein stuck

... culture stably expressing specific genes. qPCR analysis showed that there is an expression, but I have a western blot problem. I'm using polyclonal primary antibody against my ...

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by bsengez
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Topic: protein stuck
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Topic: question about shRNA knocking down
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... protein expression and activity. Western blot analysis of soluble leaf extracts using anti-ricin a-chain (RTA) ...

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Western Blotting Western blot analysis can detect one protein in a mixture of any number of proteins while ...

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by Dr.Stein
Mon Nov 28, 2005 9:02 am
Forum: Molecular Biology
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