Dictionary » W » Western blot

Western blot

western blot

(Science: technique) A technique similar to southern blotting, though it is used for proteins.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Re: Western transfer failure

... in transfer buffer. I was transferring two 12% SDS-PAGE gels to PVDF in one tank. 100V, 1 hr. One gel transferred fine, another went backwards to blot paper after 1 hr. Did I just arrange backwards? Did lack of SDS mess with electric gradient? Correlation may not be causation, so I don't claim ...

See entire post
by yanggers
Thu Feb 06, 2014 9:16 pm
 
Forum: Molecular Biology
Topic: Western transfer failure
Replies: 5
Views: 5000

Weird - shaped band on western blotting

... cell cycle regulation protein. Recently I got a weird-shaped band on my western blot. It is like an upside-down of smiling face in each lane. I use the same loading buffer, ...

See entire post
by namakulaila
Thu Oct 17, 2013 6:17 am
 
Forum: Molecular Biology
Topic: Weird - shaped band on western blotting
Replies: 2
Views: 1333

In Vitro Transcription Translation

... and autoradiographed to visulalize.. Do i have to also prove by western blot ? There are no background lysate proteins shown up in gel. Its pretty good. Any advice. ...

See entire post
by Shaanu
Mon Jun 03, 2013 7:12 am
 
Forum: Molecular Biology
Topic: In Vitro Transcription Translation
Replies: 3
Views: 1937

TCA preipitation

... of supernatant concentrated down to 100uL to detect the protein on a western blot)..How do I get 50mL of supernant? Please help. I'm so confused :cry: Thanks!! b_06er

See entire post
by biology_06er
Wed Feb 27, 2013 3:25 am
 
Forum: General Discussion
Topic: TCA preipitation
Replies: 2
Views: 2356

Protein isolation

How can you isolate any protein with Western blot? :roll: Trizol should work for protein extraction, but I have never used it. I guess they will be ...

See entire post
by JackBean
Wed Feb 13, 2013 1:13 pm
 
Forum: Molecular Biology
Topic: Protein isolation
Replies: 2
Views: 1969
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 3,126 times. 
What links here | Related changes | Permanent link