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Western

western

1. Of or pertaining to the west; situated in the west, or in the region nearly in the direction of west; being in that quarter where the sun sets; as, the western shore of France; the western ocean. Far o'er the glowing western main. (Keble)

2. Moving toward the west; as, a ship makes a western course; coming from the west; as, a western breeze. Western Church. See latin Church, under Latin. Western empire, the western portion of the roman empire, as divided, by the will of Theodosius the Great, between his sons Honorius and Arcadius, a. D. 395.


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TCA preipitation

... (im doing this on behalf of someone not here currently that they used 50mL of supernatant concentrated down to 100uL to detect the protein on a western blot)..How do I get 50mL of supernant? Please help. I'm so confused :cry: Thanks!! b_06er

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by biology_06er
Wed Feb 27, 2013 3:25 am
 
Forum: General Discussion
Topic: TCA preipitation
Replies: 2
Views: 199

Protein isolation

How can you isolate any protein with Western blot? :roll: Trizol should work for protein extraction, but I have never used it. I guess they will be inactive if is it OK for you.

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by JackBean
Wed Feb 13, 2013 1:13 pm
 
Forum: Molecular Biology
Topic: Protein isolation
Replies: 2
Views: 382

Protein isolation

... protocol may not work. I haven't heard of trizol being used for a specific protein extraction, and HIF 1 alpha seems to be favorably isolated with western blot. If anyone has any input on this, I would greatly appreciate it. Thank you.

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by roth317
Tue Feb 12, 2013 9:25 pm
 
Forum: Molecular Biology
Topic: Protein isolation
Replies: 2
Views: 382

Western Blot

Hi BIologists, I have a question, how can we know about the size of protein in the western blot? I know the full lenght, but after WB, I am seeing band with a different size! Thanks for your help.

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by Bio2013
Wed Jan 30, 2013 7:00 am
 
Forum: Cell Biology
Topic: Western Blot
Replies: 5
Views: 451

Cycloheximide-increase in protein levels???

... with cycloheximide(CHX), which is an eukaryotic translation inhibitor, through different time intervals. Then, I looked at the protein amounts by Western Blot. However, I saw that as I left them in medium containing CHX more, the levels of my protein of interest increased. How could it be possible?

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by sciencemakerinthelab
Sun Jan 27, 2013 2:56 pm
 
Forum: General Discussion
Topic: Cycloheximide-increase in protein levels???
Replies: 1
Views: 296
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