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Western

western

1. Of or pertaining to the west; situated in the west, or in the region nearly in the direction of west; being in that quarter where the sun sets; as, the western shore of France; the western ocean. Far o'er the glowing western main. (Keble)

2. Moving toward the west; as, a ship makes a western course; coming from the west; as, a western breeze. Western Church. See latin Church, under Latin. Western empire, the western portion of the roman empire, as divided, by the will of Theodosius the Great, between his sons Honorius and Arcadius, a. D. 395.


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western blotting of DNA-binding protein of mammalian cell

... i have added other sample they ran properly but not this cell lysate... what could be problem..how to get a clear SDS-PAGE and western blotting....

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by drelamuruganvet
Mon Jan 19, 2015 9:39 am
 
Forum: Molecular Biology
Topic: western blotting of DNA-binding protein of mammalian cell
Replies: 0
Views: 394

mRNA vs. cell reporter assay

I have the same question, phrased in a slightly different way: Maybe the reporter system provides us with more information than RT-PCR/Western blot, because it proves that the transcription factor is actually active too, not just present? I thought it is enough to detect a significant increase ...

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by kk
Mon Sep 08, 2014 2:57 pm
 
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 2
Views: 2902

use of a bacterial protease inhibitor cocktail with mammalia

Dear fellow biochemists, I need some advice on Western Blotting, more specifically the use of certain protease inhibitors with the RIPA cell lysis buffer and protease inhibitor cocktail. A Millipore technical representative recommended my using ...

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by rpwdoc
Tue Aug 26, 2014 10:08 pm
 
Forum: Cell Biology
Topic: use of a bacterial protease inhibitor cocktail with mammalia
Replies: 1
Views: 1928

Re: Stomach bloating and weight gain

... toxins, impurities of the system, clears up potential risks and is the number one cure for all in preventing diseases. As hard as it is to do in western civilization, with the excess at our fingertips, a fast is the much needed answer for many. It also utilizes the stored fat and the energy is ...

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by Sydstatue
Mon Aug 11, 2014 6:17 am
 
Forum: Physiology
Topic: Stomach bloating and weight gain
Replies: 453
Views: 2911352

Citing articles and copyright

... if I do not cite articles in the official way? 2. If I find a complete article in the PubMed Central, but the article was originally published in Western Journal of Medicine, am I allowed to link link to PubMed Central? Example: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1071161/

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by boron
Sat Aug 09, 2014 5:40 pm
 
Forum: General Discussion
Topic: Citing articles and copyright
Replies: 2
Views: 1706
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