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Wells, G

(Science: person) 20th century British dermatologist.

See: wells' syndrome.

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centrifugation of hemocytes

... so because many authors used it toward such cells). Even if the cells were lysed, i expect them to be "pelleted" to the bottom of the wells and so when i wash, the cells should stay sticked, unless i resuspend with PBS containing EDTA or heparin... Is it correct? When i take off the ...

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by Thom
Tue Jan 28, 2014 10:50 am
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 2602

question about G418 sceening test

... ug/mL serie. It has been reported that 700 ug/mL was used for G418 screening for Huh-7. However, after 3 days incubation, all my cells in all wells were dead. I have no idea why is that. One thing I noticed is that my Huh-7 grow really fast, it will take just 2-3 days to reach full confluency. ...

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by cavaliersun2003
Mon Nov 11, 2013 7:28 am
Forum: Cell Biology
Topic: question about G418 sceening test
Replies: 0
Views: 1030

electrophoretic mobility shift assay (EMSA)

... Do anyone have a good tip what went wrong? (I use 6% non-danaturing PAA gel without a stacking gel, I pre-run it at 100V for 1h, I rinse the wells before loading, I don't vortex the samples, the loading buffer doesn't contain SDS either)

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by kk
Fri Nov 08, 2013 12:21 pm
Forum: Molecular Biology
Topic: electrophoretic mobility shift assay (EMSA)
Replies: 1
Views: 1218

aflatoxin screening Test and peaunt allergen screening test

aflatoxin screening Test what substance is found in the red wells and the clear wells provided? Ans : red well is enzyme and clear wells is antibody? <---- is that right peaunt allergen screening test what substance is found in the control provided ...

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by Damian2012
Fri Oct 04, 2013 5:11 pm
Forum: Human Biology
Topic: aflatoxin screening Test and peaunt allergen screening test
Replies: 2
Views: 834

Fixing CFSE labeled cells

... / reliable? Or does fixation / storage affect the signal? And another thing, how sensitive is the CFSE signal to light? Do I have to wrap my 6-wells plate in aluminium foil? greets, Menno

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by BiolMC
Tue Jun 11, 2013 12:34 pm
Forum: Cell Biology
Topic: Fixing CFSE labeled cells
Replies: 0
Views: 724
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