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Weigert's stain

(Science: technique) 1. For actinomyces, a staining method using immersion in a dark red orsellin solution in alcohol, then staining in crystal-violet solution.

See: iron haematoxylin.

2. For elastin, a staining solution of fuchsin, resorcin, and ferric chloride; elastic fibres stain blue-black.

3. For fibrin, a staining method using solutions of aniline-crystal violet and iodine-potassium iodide, then decolorizing in aniline oil and xylol; the fibrin is stained dark blue.

4. For myelin, a staining method using ferric chloride and haematoxylin; myelin stains deep blue, degenerated portions a light yellowish colour.

5. For neuroglia, a complicated process in which the final treatment is like that for staining fibrin; neuroglia and nuclei stain blue.

6. For reticulum, a silver impregnation technique in which reticulum appears as black, well-defined fibres without beading and with a relatively clear background.