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Wavelength

wavelength

(Science: radiobiology) The length of a single cycle of a wave, usually measured from crest-to-crest. For electromagnetic waves, the wavelength determines the type (radio, infrared, visible, ultraviolet, x-Ray, gamma-ray) of radiation, in the case of visible light, wavelength determines the colour of the light. The distance (measured in the direction of propagation) between two points in the same phase in consecutive cycles of a wave.A measurement between two successive points of a wave, which can be used to measure things like light and sound. The wavelength of lights' visible to the human eye range from violet (400 nanometres) to red (750 nanometres).


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Re: blank and substrate control give OD reads

Is there anything in the buffer that absorbs light at the wavelength you are using for your OD readings? It might be worth running the buffer in a spectrophotometer and comparing against water at wavelengths near your OD measurement. Dear jonmoulton, ...

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by intandiana
Sat Jan 18, 2014 8:39 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 3400

Re: blank and substrate control give OD reads

Is there anything in the buffer that absorbs light at the wavelength you are using for your OD readings? It might be worth running the buffer in a spectrophotometer and comparing against water at wavelengths near your OD measurement.

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by jonmoulton
Thu Jan 16, 2014 3:23 pm
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 3400

Metabolic Processes (Photosynthesis)

... feel I have exhausted my options would be awesome if you guys could give me a hand. 18.What is the relationship between the numerical value of the wavelength of light and energy? 24.How can knowledge of atomic structure help you understand metabolic reactions ( refer to photosynthesis ETC ) Explain ...

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by Ninthprince
Tue Oct 22, 2013 1:38 am
 
Forum: Botany Discussion
Topic: Metabolic Processes (Photosynthesis)
Replies: 1
Views: 3409

Re: Resonance transfer

... molecule (fluorochrome) is excited with light; for now, let's say it's blue light. Instead of simply emitting the excitation energy at a longer wavelength, let's say green light, it instead passes the energy (the "exciton") to another fluorochrome that has an excitation energy matching ...

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by jonmoulton
Fri Jun 07, 2013 3:20 pm
 
Forum: General Discussion
Topic: Resonance transfer
Replies: 1
Views: 1172

Two-color in situ help

... did not seem to factor in either since the anti-DIG + cy3 always worked. I thought of trying cy5 but we do not seem to have the filter for that wavelength...so i m in quite a pickle right now...

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by vastgenome
Thu Feb 14, 2013 11:59 pm
 
Forum: Molecular Biology
Topic: Two-color in situ help
Replies: 3
Views: 2208
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