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Wavelength

wavelength

(Science: radiobiology) The length of a single cycle of a wave, usually measured from crest-to-crest. For electromagnetic waves, the wavelength determines the type (radio, infrared, visible, ultraviolet, x-Ray, gamma-ray) of radiation, in the case of visible light, wavelength determines the colour of the light. The distance (measured in the direction of propagation) between two points in the same phase in consecutive cycles of a wave.A measurement between two successive points of a wave, which can be used to measure things like light and sound. The wavelength of lights' visible to the human eye range from violet (400 nanometres) to red (750 nanometres).


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Two-color in situ help

... did not seem to factor in either since the anti-DIG + cy3 always worked. I thought of trying cy5 but we do not seem to have the filter for that wavelength...so i m in quite a pickle right now...

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by vastgenome
Thu Feb 14, 2013 11:59 pm
 
Forum: Molecular Biology
Topic: Two-color in situ help
Replies: 3
Views: 253

solutions containing different concentrations of oxaloacetat

... 250 μL NADH ---- Dilution of 1:10 OAA: 900 μL water + 100 μL OAA malate: 900 μL water + 100 μL malate NADH: 900 μL water + 100 μL NADH we set the wavelength of the spectrophotometer to 340 nm. used a cuvette with 1 mL water as black, and them measured the absorbance of each of the dilutions of ...

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by ladwer
Sun Oct 07, 2012 2:38 am
 
Forum: General Discussion
Topic: solutions containing different concentrations of oxaloacetat
Replies: 1
Views: 495

Re: Is protein degrade by UV ?

It will depend on the wavelength of the UV light and its intensity. Aromatic amino acids, such as tyrosine, can absorb shorter-wavelength UV and become reactive. I found a lot of relevant information by searching the keyword group "UV ...

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by jonmoulton
Fri Aug 31, 2012 4:04 pm
 
Forum: Cell Biology
Topic: Is protein degrade by UV ?
Replies: 3
Views: 1936

Lowry method's wavelength choice

The best way is to take the wavelength with maximum absorbance. But sometimes there may be some interference and in such case you will need to choose another wavelength.

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by JackBean
Fri Jul 13, 2012 9:30 am
 
Forum: Molecular Biology
Topic: Lowry method's wavelength choice
Replies: 1
Views: 842

Lowry method's wavelength choice

... best protocol for the method, that is, I found the assay's wavelegth usually used lie from 500 - 750 nm. What is the consideration of choosing the wavelength, anyway. Will the result be different if I use different wavelength. I should have some reasons of taking such wavelength of course. I'm ...

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by faredeiss
Tue Jul 10, 2012 4:17 pm
 
Forum: Molecular Biology
Topic: Lowry method's wavelength choice
Replies: 1
Views: 842
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