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Water bath

water bath

In chemistry, a vessel containing water, in which a container holding a substance to be heated or evaporated can be immersed.

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Re: sterilization in microwave oven

... or too expensive for the purpose. Nurses used to use a boiling water bath to 'sterilize' needles for reuse and to 'sterilize' surgical instruments. It is innocence ...

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by fdgsr
Tue Mar 18, 2014 3:13 pm
Forum: Microbiology
Topic: sterilization in microwave oven
Replies: 29
Views: 93696


the incubation time for fecal coliform in a 44.5deg. water bath is 24 hour.what if the incubation happend at 48 hrs? is there any effect in the result?

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by michimichi
Thu Jan 26, 2012 2:43 am
Forum: Microbiology
Topic: incubation
Replies: 1
Views: 541

Re: Western Blot TroubleShooting

... supernatant. I add 2X SDS PAGE loading dye and heat at 100C in a sand bath for 10 min. I run my samples on a SDS-PAGE gel (7.5%) for 45- 1 hour ... I use a solution that is transblot solution, methanol+ distilled water. I let my gel sit in the transblot solution for 15 min before I start ...

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by mutant11
Mon Nov 21, 2011 3:11 am
Forum: Molecular Biology
Topic: Western Blot TroubleShooting
Replies: 7
Views: 2696

Re: hydroxyproline assay to determine collagen content

... are combined and the final volume is brought to 10 ml with distilled water. Make sure the pH is 6.0. Store in the refrigerator with aluminum foil ... may be expedited by heating on a hot plate) then placed in a 60C water bath for 20 min (at this point observe the colorimetric change of the solutions ...

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by ucsfmed2012
Thu Oct 27, 2011 9:30 am
Forum: Molecular Biology
Topic: hydroxyproline assay to determine collagen content
Replies: 5
Views: 6765

Protoplast Isolation

... - This is made by adding 13 grams of sorbitol powder to 100cm3 of water, is it not? 21% Sucrose Solution - This is made by adding 21 grams ... sorbitol solution in a test tube. 3. Incubate the test tube in a water bath, set at 37C, for 5 minutes. <-- How accurate does the temperature ...

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by ev350
Thu Sep 08, 2011 8:23 pm
Forum: Cell Biology
Topic: Protoplast Isolation
Replies: 2
Views: 5782
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