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Wash-bottle

wash-bottle

A bottle with a tube passing to the bottom, through which gases are forced into water to purify them, a stoppered bottle with two tubes, one ending above and the other below a fluid, so that air blowing through the short tube forces liquid in a small stream from the free end of the long one; used for washing chemical apparatus.


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Re: centrifugation of hemocytes

... I gently took off the medium and resuspend the cells by pipetting thoroughly up/down: the viability worked well. However, that time i skiped the washing step (and wait 24h...), which can be annoying when working with different dyes... I will try to wash gently and gently take off the fresh-washing-PBS ...

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by Thom
Tue Jan 28, 2014 4:16 pm
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
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Re: centrifugation of hemocytes

Before you wash the cells, can you see any pellet? Wash gently, add your final PBS and pipet to resuspend. Your protocol sounds good to me.

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by jonmoulton
Tue Jan 28, 2014 3:53 pm
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
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centrifugation of hemocytes

... used it toward such cells). Even if the cells were lysed, i expect them to be "pelleted" to the bottom of the wells and so when i wash, the cells should stay sticked, unless i resuspend with PBS containing EDTA or heparin... Is it correct? When i take off the medium after centrifugation, ...

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by Thom
Tue Jan 28, 2014 10:50 am
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
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