W procedure

Hi all, I'm planning to use alizarin red staining assay to observe and quantify bone formation on my scaffolds.. Will it be the same procedure as being done on a plate/well. For quantification purpose, we use the de-staining solution and take results using a plate reader. Is it necessary ...

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I have been given a batch of XL1 blue cells. I made them competent using calcium chloride procedure. To test them, i plated them out on agar plate containing: Kanamycin, Tetracyclon, Ampicilin an control. After overnight incubation, there is bacteria colonies in all ...

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... out of its environment and put it in a new one. If it changes phenotype, then you have your answer. (I'm simplifying the necessary experimental procedure a bit, of course.)

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... troubles with the isolation of the bands. I ran the experiment a number of times and didn't always get the same result (despite the exact same procedure and minimal scope for human error). My supervisor suggested that this could be something to do with the splicing nature of the gene.... he ...

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... 20000. The only way I know to do it is to download FASTA, reformat in notepad or word then use CLUSTALW. However, this would be a very lenghtly procedure . Are there any online free (preferably) or other alignment tools that would do the job with accession numbers ony? Any advise is much appreciated.

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