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W factor

W factor --> biotin

(Science: biochemistry) A prosthetic group for carboxylase enzymes. Important in fatty acid biosynthesis and catabolism and has found widespread use as a covalent label for macromolecules which may then be detected by high affinity binding of labelled avidin or streptavidin. Essential growth factor for many cells.


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mRNA vs. cell reporter assay

... different way: Maybe the reporter system provides us with more information than RT-PCR/Western blot, because it proves that the transcription factor is actually active too, not just present? I thought it is enough to detect a significant increase in the expression by Western blot and we could ...

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by kk
Mon Sep 08, 2014 2:57 pm
 
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 2
Views: 405

staphylococcus aureus and biofilm formation

... enzyme ( eg. Beta lactamase) or other mechanism against antibiotic. I think normal microflora of gut form biofilm. Biofilm formation isn't a sole factor to confer antibiotic resistance.

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by josem
Fri Aug 01, 2014 7:53 pm
 
Forum: Microbiology
Topic: staphylococcus aureus and biofilm formation
Replies: 3
Views: 3004

Oakley Files Patent overtrædelse retssag mod Nike nike til

... free 2.0</a> Inc. (NYSE: NKE NKE Nike, Inc. (aktiesymbolet) NKE Nationalt Videnscenter eksamen tilbud nike free (Canada) NKE NK2 homeobox Factor Bindende ElementNKE Northwest Kodly EducatorsNKE Network Kernel Extension). Den Dragten hævder, at Nike Max Lens teknologi, figurerede i hele ...

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by FosterEa
Wed Jul 16, 2014 12:04 am
 
Forum: Human Biology
Topic: Electromagnetic fields generated by the human body
Replies: 40
Views: 161403

mRNA vs. cell reporter assay

Hi, I have a GFP reporter cell line that drives GFP expression upon activation of a transcription factor. In theory, I can study transcription factor activation via the GFP signal, or by measuring the mRNA of the transcription factor. I wonder what are the pros and cons ...

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by aguy
Mon Jul 07, 2014 2:50 am
 
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 2
Views: 405

Re: How did endosymbionts coordinate replication?

Regarding the regulation of replication of mitochondria, this paper discusses an influence of tumor necrosis factor alpha on mitochondrial replication while exploring the effect of adenosine on that regulatory system. http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0098459 ...

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by jonmoulton
Wed Jun 11, 2014 2:08 pm
 
Forum: Cell Biology
Topic: How did endosymbionts coordinate replication?
Replies: 9
Views: 4429
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