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Vortex

vortex

Origin: L. Vortex, vertex, -icis, fr. Vortere, vertere, to turn. See Vertex.

1. A mass of fluid, especially of a liquid, having a whirling or circular motion tending to form a cavity or vacuum in the center of the circle, and to draw in towards the center bodies subject to its action; the form assumed by a fluid in such motion; a whirlpool; an eddy.

2. A supposed collection of particles of very subtile matter, endowed with a rapid rotary motion around an axis which was also the axis of a sun or a planet. Descartes attempted to account for the formation of the universe, and the movements of the bodies composing it, by a theory of vortices.

3. (Science: zoology) Any one of numerous species of small turbellaria belonging to Vortex and allied genera.

(Science: chemistry) Vortex atom, a hypothetical ring-shaped mass of elementary matter in continuous vortical motion. It is conveniently regarded in certain mathematical speculations as the typical form and structure of the chemical atom. Vortex wheel, a kind of turbine.


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electrophoretic mobility shift assay (EMSA)

... what went wrong? (I use 6% non-danaturing PAA gel without a stacking gel, I pre-run it at 100V for 1h, I rinse the wells before loading, I don't vortex the samples, the loading buffer doesn't contain SDS either)

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by kk
Fri Nov 08, 2013 12:21 pm
 
Forum: Molecular Biology
Topic: electrophoretic mobility shift assay (EMSA)
Replies: 1
Views: 1144

Real time PCR

make sure to vortex your DNA samples before you mix them for the qPCR in order to generate the std curve. also make sure you measured the DNA accurately to make the std curve calculation, for example I found out that checking by ...

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by bravebeaker
Fri Oct 19, 2012 6:26 am
 
Forum: Molecular Biology
Topic: Real time PCR
Replies: 4
Views: 2206

Re: ligation problem although try everything,suggestions?

Vortex the buffer, not the enzyme.

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by wbla3335
Wed May 23, 2012 7:59 pm
 
Forum: Molecular Biology
Topic: ligation problem although try everything,suggestions?
Replies: 7
Views: 4046

Centrifuging low amount of bacteria

... the side of the tube and place it in the centrifuge so your pellet would be expected to be on the line. Carefully transfer old media to new tube. Vortex that media (to resuspend pellet if you transferred it accidentally) and check it for bacteria. If nothing is there, than you pellet is in the ...

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by Cat
Sat Dec 10, 2011 5:48 pm
 
Forum: Microbiology
Topic: Centrifuging low amount of bacteria
Replies: 5
Views: 4151

Problem with transgenic mice

... You are using cDNA - does this mean a crude RT prep? You could do a DNA clean up to remove any proteins (a 50:50 Phenol/chloroform say 100-500ul , vortex, spin 10mins, pipette of the aqueous phase to a fresh tube. dispose of phenol carefully according to local rules (evap in a chemicals fumehood ...

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by merv
Sun Oct 02, 2011 9:32 pm
 
Forum: Genetics
Topic: Problem with transgenic mice
Replies: 2
Views: 6998
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