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Volumes

volume substitute

infusion of cell-free or volume-expanding fluids such as dextran for replacement of fluid lost from the circulation as part of the prevention or treatment of circulatory shock.


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Deproteinizing plasma?

Hello! Here is a protocol currently used by a core facility for the deproteinization of small volumes of mouse plasma for measurement of plasma radiolabeled glucose: 1) Add 10 uL of plasma into 20 uL of dd water. 2) Add 25 uL of BaOH (0.3N from Sigma B4059) and then add ...

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by ChristopherC4rm34n
Tue Sep 09, 2014 6:15 pm
 
Forum: Physiology
Topic: Deproteinizing plasma?
Replies: 10
Views: 9461

Nuclear protein purification

cV = cV right? So, you'll have: 2M . V1 = 5M . V2 2M/5M = V2/V1 -> ratio of original (5M stock) and final (your solution) volumes is 2 : 5. In other words, the 5M solution will make 2 parts of of 5 parts of 2M solution. Thus the solution to which you're adding the NaCl will make 3 ...

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by JackBean
Mon Apr 15, 2013 9:36 am
 
Forum: Molecular Biology
Topic: Nuclear protein purification
Replies: 4
Views: 1740

Using the pipette

Since your pipette is 50-200ul you should be able to adjust the setting to 100ul volumes. Assuming everything is calibrated you'll get accurate results. Also it helps to use tips that have graduation marks...usually a line on the tip that marks 10ul, 50ul, 100ul...etc.

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by nestaB
Tue May 22, 2012 11:25 pm
 
Forum: Cell Biology
Topic: Using the pipette
Replies: 4
Views: 2481

Re: glass cuvette cleaning

... to clean the cuvette. The rinsing is critical -- get all the water off the cuvette between rinses and I'd do at least five rinses. Using smaller volumes of rinse water and repeating the rinse is more effective than using larger rinse volumes fewer times. Between most measurements, rinsing with ...

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by jonmoulton
Tue Mar 06, 2012 4:50 pm
 
Forum: General Discussion
Topic: glass cuvette cleaning
Replies: 2
Views: 3394

Re: counting cells

... 10 fold still to many..15 fold seemed the next logical factor. I was mixing in a well before loading on to a hemocytometer so I just used useable volumes

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by biology_06er
Mon Jan 30, 2012 8:25 pm
 
Forum: Cell Biology
Topic: counting cells
Replies: 3
Views: 1545
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