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(Science: physics) electric potential or potential difference, expressed in volts.

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Re: Depolarization = ion channel opening?

I think you are correct (or more precisely when the Na+ channels open since the K+ open later). There is a threshold voltage that switches the state of what I found to be a flip-flop. The circuit is also called a "monostable multivibrator".

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by GaryGaulin
Thu Jan 08, 2015 6:01 am
Forum: Cell Biology
Topic: Depolarization = ion channel opening?
Replies: 1
Views: 658

Too many ions inside a neuron cell. What happens?

For example, there are diseases where the sodium channel cannot gauge voltage difference effectively, and so it remains open, rapidly building up a concentration of sodium ions in the cell, and this disorder often affects potassium channels, so there is practically ...

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by Coelacanth
Tue Dec 03, 2013 1:34 am
Forum: General Discussion
Topic: Too many ions inside a neuron cell. What happens?
Replies: 1
Views: 2399

Patch-Clamping - Need help in basics

... data, and it looks like my brain refuses to get it. So, my (probably VERY basic) question is now: I have a cell in whole cell patch-clamp and do a voltage ramp. How do I have to interpret the measurement? I have, as a result, a set of curves given in pA. Does a negative number indicate that cations ...

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by Jennileinchen
Mon Jul 22, 2013 3:22 pm
Forum: Molecular Biology
Topic: Patch-Clamping - Need help in basics
Replies: 0
Views: 1147

Asking for help with electrophoresis

... of using microwave, this time I used a heating block with stirring. 3. Used a much bigger electrophoresis tank with buffer circulation. The voltage was decreased to 2~3V/cm. It is hard to say which was the critical change, but the overall result was good. My main targets are 200bp~400bp, ...

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by ll2013
Mon Jun 17, 2013 1:51 pm
Forum: Molecular Biology
Topic: Asking for help with electrophoresis
Replies: 5
Views: 3339

DNA extraction from Human blood

... Probably you overloaded (too much DNA) and thus the wierd appearance of your bands. Try to load little bid less of sample and use lower voltage. stain the gel after electrophoresis, not before.

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by triton
Thu Sep 13, 2012 11:12 am
Forum: Molecular Biology
Topic: DNA extraction from Human blood
Replies: 3
Views: 2725
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