
|
|
Dictionary » V » Vmax VmaxDefinition The maximum initial velocity or rate of a reaction.
In enzyme kinetics, Vmax is the maximum velocity or rate at which the enzyme catalyzed a reaction. It happens when all enzyme active sites are saturated with substrate. Since the maximum velocity is described to be directly proportional to enzyme concentration, it can therefore be used to estimate enzyme concentration. ![]()
Please contribute to this project, if you have more information about this term feel free to edit this page ![]()
Results from our forumRe: Lab of Vmax and KMThat's it. The velocity approaches Vmax asymptotically as the substrate concentration increases. However, changing other conditions can change the Michaelis–Menten plot -- for instance, a different pH or a different temperature can lead to ...
See entire post
Lab of Vmax and KM... I recorded 28 points with [S] (mM) and V (mM/min). I subsequently plotted two graphs (a Michaelis-Menten and Lineweaver burk) and calculated the Vmax and Km. Vmax = 0.005033 mM/min KM = 23.41 So that I completely understand: the maximum velocity this enzyme can react at is 0.005022 mM/min at ...
See entire post
Enzyme kinetics... by plotting the 1/[V] by 1/[S] graph, we may find the intercept on y axis....which is 1/Vm in this case....and then do the reciprocal to find out Vmax.....and then put this value in the above equation.....at a particular [S] and v....and find the value of Km....:)
See entire post
Noncompetitive inhibition and allosteric inhibitionThe two terms overlap to some extent. All "non-competitive" means is that the inhibitor lowers Vmax without changing Km. You can't read much mechanism into it. The notion of competitive, non-competitive, mixed-competitive are all kinetic behaviors. An allosteric site ...
See entire post
Enzyme kineticsSorry, that should be X -intercept = -1/Km, not y-intercept. And the slope is Km/Vmax not Vmax. Memory didn't serve me very well this time and I was too lazy to derive it from the rate expression-though that's a useful excercise if you've never done it before. This ...
See entire post
This page was last modified 09:28, 19 July 2008. This page has been accessed 9,190 times. |
© Biology-Online.org. All Rights Reserved.
Register | Login
| About Us | Contact Us | Link to Us | Disclaimer & Privacy
Science Network - Braintrack.com - University Directory | Chemicool.com - Chemistry