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Vectors

(noun) Plural form of vector.

See vector for definition and additional information.


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Gateway technology

The point is that you get your gene into an universal vector from which you can easily transfer it into variety of destination vectors without any need of further PCR as PCR is often troublesome and introduces mutations.

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by JackBean
Wed Aug 20, 2014 6:42 pm
 
Forum: Molecular Biology
Topic: Gateway technology
Replies: 1
Views: 407

Gateway technology

From what I understand, the gateway technology is a lab method to obtain cloning and expression vectors based on the property of phages to recombine its genetic material with bacterias. However, why do I need to perform the BP reaction, if I want to obtain an expression ...

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by miibay
Tue Aug 19, 2014 10:47 pm
 
Forum: Molecular Biology
Topic: Gateway technology
Replies: 1
Views: 407

cDNA cloning and Lentiviral transfection

... gene, to allow you to amplify it in bacteria. I don't know what your vector is, so I can't say if you can or cannot (you probably can, most vectors are designed like that)

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by BlahBlahBlah
Fri Jul 12, 2013 1:09 pm
 
Forum: Molecular Biology
Topic: cDNA cloning and Lentiviral transfection
Replies: 1
Views: 867

Producing unknown protein

... sequence which is unknown, when we checked any databases we see that it is probably new. the aim is how we can make this protein produced via vectors using molecular approaches? What can be experimental design.

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by drfuture
Sat May 18, 2013 8:43 pm
 
Forum: Microbiology
Topic: Producing unknown protein
Replies: 2
Views: 2051

QRS complex

Go back to looking at physics of vectors of electrical charge. Vectors never cross and they are vectors that can be summed based on their components. If you think of the heart, electricity flows from the SA node, through the AV node, and ...

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by daniel.kurz
Tue Mar 19, 2013 1:29 pm
 
Forum: Physiology
Topic: QRS complex
Replies: 2
Views: 4761
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