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Dictionary » V » Vector VectorDefinition noun, plural: vectors (1) (epidemiology) An organism or vehicle that transmits the causative agent or disease-causing organism from the reservoir to the host. (2) (molecular biology) A vehicle (e.g. a plasmid) used to transfer the genetic material such as DNA sequences from the donor organism to the target cell of the recipient organism. (3) (biology) A biotic agent that disperses reproductive structures of another organism, as a bee transmitting pollen to the stigma of a flower. (4) (mathematics) A term describing something with both direction and magnitude.
In molecular biology, a vector may refer to a plasmid vector, a binary vector, a cloning vector, a shuttle vector, a viral vector, or an expression vector.
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Results from our forumHow can I add a kozak sequence in plasmid pMT/bip/V5-His Athe Kozak sequence is around the START codon, so you've had to play with the vector. But it's probably normally working if they sell it in Life Technologies.
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Inserting cDNA into a retroviral vector?..sure that it done on regular basis. ut depends what cells you want to insert it into, what vector to use, etc.
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Inserting cDNA into a retroviral vector?..Thank you, but maybe there's another option: could I make the cDNA of the retrovirus using RT-PCR, then insert the cDNA of the gene into the vector, and later insert that vector into dividing cells of another organism? Theoretically, it's the retroviral cDNA that inserts itself into the host ...
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Inserting cDNA into a retroviral vector?..Hello everyone. Let's say I have a retroviral vector and its genome consists of single stranded RNA. Is it possible to insert double stranded cDNA into this vector? If yes, what are the processes that need to happen in order to make this occur? ...
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ligation errorhello and good day ! i have done ligation of xylanase gene into pRSET B and pACYCDUET-1 vector next , i incubate onto luria bertani (i use E. coli DH5a for host cell ) with antibiotic (amp50 for pRSET B, cam30 for pACYCDUET-1) at 37'C overnight. on the next day, i found ...
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