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Vector

Definition

noun, plural: vectors

(1) (epidemiology) An organism or vehicle that transmits the causative agent or disease-causing organism from the reservoir to the host.

(2) (molecular biology) A vehicle (e.g. a plasmid) used to transfer the genetic material such as DNA sequences from the donor organism to the target cell of the recipient organism.

(3) (biology) A biotic agent that disperses reproductive structures of another organism, as a bee transmitting pollen to the stigma of a flower.

(4) (mathematics) A term describing something with both direction and magnitude.


Supplement

In molecular biology, a vector may refer to a plasmid vector, a binary vector, a cloning vector, a shuttle vector, a viral vector, or an expression vector.


Word origin: Latin, from vehere, carrier. Related form(s): vectorial (adjective)

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Results from our forum


Help: Confused with primers in a PCR of a vector?

1) positive control (T7-gene-T3). 2) if your gene is inserted into the vector so the beginning of the gene is right next to T7: T7-gene. If your gene is inserted into the vector so that the beginning of the gene is next to T3: Nothing since both primers bind ...

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by poobear
Fri Apr 11, 2014 9:58 pm
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 2152

Help: Confused with primers in a PCR of a vector?

... being introduced to a lot of different lab techniques, so I'm not entirely familiar with what is going on. We recently ligated a gene into a pBS vector and popped it into some E. coli cells. We looked for positive clones via blue/white screening and isolated/purified the respective plasmid. Then, ...

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by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 2152

Re: Gene cloning question

... sequence is CA/TATG, My question, is The ATG of Nde1 act as a start codon in result I must delete The ATG of my sequence or I can use the ATG of vector in Nde1 site as a start codon? (NOTE: My gene is eukaryotic) Is there anyone make a sugestion?

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by mohajeri
Thu Feb 06, 2014 10:01 pm
 
Forum: Molecular Biology
Topic: Gene cloning question
Replies: 20
Views: 17420

Cell and Molecular Revision Question Help!!!

except of the two ends, it seems OK. Both vector and insert have two ends so you do not need to include that ;)

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by JackBean
Thu Oct 03, 2013 1:10 pm
 
Forum: Molecular Biology
Topic: Cell and Molecular Revision Question Help!!!
Replies: 4
Views: 2568

Re: Cell and Molecular Revision Question Help!!!

This is what I have.. Consider the Vector (Mass of Vector): pBR328 (4.9 x 103) x 660 = 2.94x106 Daltons Given: Moles = Mass/MW Moles = 50 x 10-9g / 2.94x106 Moles = 17 x10-15 Each vector has two ends therefore x2 = x2 Therefore there are 34 ...

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by Didact1
Mon Sep 30, 2013 10:02 pm
 
Forum: Molecular Biology
Topic: Cell and Molecular Revision Question Help!!!
Replies: 4
Views: 2568
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