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Vector

Definition

noun, plural: vectors

(1) (epidemiology) An organism or vehicle that transmits the causative agent or disease-causing organism from the reservoir to the host.

(2) (molecular biology) A vehicle (e.g. a plasmid) used to transfer the genetic material such as DNA sequences from the donor organism to the target cell of the recipient organism.

(3) (biology) A biotic agent that disperses reproductive structures of another organism, as a bee transmitting pollen to the stigma of a flower.

(4) (mathematics) A term describing something with both direction and magnitude.


Supplement

In molecular biology, a vector may refer to a plasmid vector, a binary vector, a cloning vector, a shuttle vector, a viral vector, or an expression vector.


Word origin: Latin, from vehere, carrier. Related form(s): vectorial (adjective)

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STR analysis transfected cells

I stably transfected a population of MCF-7 cells with a pcnda Her2 vector ( I'll call these MCF7-Her2) and now just need to compare my derived cell line with the parent MCF-7 cells by microsatellite STR analysis. Since it is just an addition of one gene, ...

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by kingmcb
Thu Aug 21, 2014 11:13 pm
 
Forum: Molecular Biology
Topic: STR analysis transfected cells
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Views: 346

Gateway technology

The point is that you get your gene into an universal vector from which you can easily transfer it into variety of destination vectors without any need of further PCR as PCR is often troublesome and introduces mutations.

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by JackBean
Wed Aug 20, 2014 6:42 pm
 
Forum: Molecular Biology
Topic: Gateway technology
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Gateway technology

From what I understand, the gateway technology is a lab method to obtain cloning and expression vectors based on the property of phages to recombine its genetic material with bacterias. However, why do I need to perform the BP reaction, if I want to obtain an expression ...

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by miibay
Tue Aug 19, 2014 10:47 pm
 
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Help: Confused with primers in a PCR of a vector?

1) positive control (T7-gene-T3). 2) if your gene is inserted into the vector so the beginning of the gene is right next to T7: T7-gene. If your gene is inserted into the vector so that the beginning of the gene is next to T3: Nothing since both primers bind ...

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by poobear
Fri Apr 11, 2014 9:58 pm
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
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Views: 2687

Help: Confused with primers in a PCR of a vector?

... being introduced to a lot of different lab techniques, so I'm not entirely familiar with what is going on. We recently ligated a gene into a pBS vector and popped it into some E. coli cells. We looked for positive clones via blue/white screening and isolated/purified the respective plasmid. Then, ...

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by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
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