Validation

I use qPCR for validation of CNVs detected by array technology. Pfaffl method is used for calculations (delta-delta Ct method corrected for PCR effeciency). In theory in case of deletion the ratio target/reference must be 1/2=0.5, ...

See entire post

Thanks for the reply jack. Are these observations considered to provide empirical validation for macroevolution? What birds + plants are you referring to?

See entire post

... of length more than 20 amino acids and having start codon, stop codon in the same frame) which have not been reported as genes (non-genes). The validation set has 1000 known genes and 1000 non-genes from E coli .K-12 distinct from those used in the training set. The test set contains another ...

See entire post

... it should work as well as PCA but it depends on the material being cultured. It's probably not good for water and there's probably not much if any validation work for other materials.

See entire post

... it says: Exactly what facts should I check? The wiki article simply cited its reference to what leaky guts is purported to be. This is in no way a validation that leaky gut is a legitimate illness recognized by the medical community. Consider the source you cited, a Herbal supplement supplier. ...

See entire post