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V gene

V gene --> variable gene

v region


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Gateway technology

The point is that you get your gene into an universal vector from which you can easily transfer it into variety of destination vectors without any need of further PCR as PCR is often troublesome and introduces mutations.

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by JackBean
Wed Aug 20, 2014 6:42 pm
 
Forum: Molecular Biology
Topic: Gateway technology
Replies: 1
Views: 36

Gateway technology

... I understand, the gateway technology is a lab method to obtain cloning and expression vectors based on the property of phages to recombine its genetic material with bacterias. However, why do I need to perform the BP reaction, if I want to obtain an expression clone of a certain gene? Why can't ...

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by miibay
Tue Aug 19, 2014 10:47 pm
 
Forum: Molecular Biology
Topic: Gateway technology
Replies: 1
Views: 36

How is Mycoplasma a gram positive bacteria

... in the phylum Firmicutes, consisting of low G+C Gram-positive bacteria such as Clostridium, Lactobacillus, and Streptococcus based on 16S rRNA gene analysis.

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by erangasts2
Thu Aug 14, 2014 12:25 am
 
Forum: Microbiology
Topic: How is Mycoplasma a gram positive bacteria
Replies: 2
Views: 2683

Question about hair color and tallness inheritance

Hello there! So i dont know much about genetics yet, but im very open minded about it. The situation is my father has dark brown hair and he had a wife back then and they made two children but they divorced. Both of the children have ginger ...

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by hitom
Thu Aug 07, 2014 9:58 pm
 
Forum: Genetics
Topic: Question about hair color and tallness inheritance
Replies: 0
Views: 154

Looking for a low "dead volume" syringe

Hopefully this is the right area for this post, but I will be performing a gene therapy mouse experiment for which we will be injecting mice with our viral construct. Unfortunately, the syringe we are using has a dead volume of about 60ul which adds up to quite ...

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by jsil
Wed Aug 06, 2014 6:45 pm
 
Forum: General Discussion
Topic: Looking for a low "dead volume" syringe
Replies: 0
Views: 224
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