V gene

... BglII and XbaI). Because there's a Bip signal sequence ahead of the enzyme site BglII, so I CAN NOT add Kozak sequence in the N-Term of my scfv gene, is that right? So where should I add the Kozak sequence? Thanks for your time. Alex.

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Thank you, but maybe there's another option: could I make the cDNA of the retrovirus using RT-PCR, then insert the cDNA of the gene into the vector, and later insert that vector into dividing cells of another organism? Theoretically, it's the retroviral cDNA that inserts itself into the ...

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... by Jorde, Carey and Bamshad: Consider, for example, a dominant disease that results in death before the person can reproduce. This is termed a genetic lethal mutation because, even though the individual might survive for some time, he or she contributes no genes to the next generation. Each ...

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Hello, I am using transgenic mouse where my gene of interest is floxed. When I cross this mouse line with Tamoxifen inducible Cre line, CAGGCre-ERTM, I get mice with with heterozygous Cre and floxed gene of interest. The experimental plan was to ...

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... I know next to nothing about homology, so I can't speak on that any further. The 'theory' of 'evolution' again is not a 'theory' (not change in gene frequency, or any change in any living thing ever). No it can never be 'falsified' because 1) Its not science to begin with 2) Anything on earth ...

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