V antigen

... a fluorescent tag to detect the first antibody. The two-antibody approach is cheaper than making a custom-labelled fluorescent antibody for every antigen you need to detect. For instance, the first antibody (called the primary antibody) might be raised in a rabbit, while the second antibody (the ...

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Kumar dude you can perfectly google this or even better, google imaging so 3 ways antibody work 1. antibody directly inhibit antigen google image will answer you 2. recruit complements google image 3. recruit those big cells that eat the antigens up. google image

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How does antibodies neutralize the action of antigens?

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Oh yeah, forgot to add, if you are using antigen reagent from a supplier, did you use the same supplier pre and post? Also the same lot number of antigen reagent? Small variability in antigens can sometimes occur with new reagent or between lots ...

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Could it be that the maximum amount of antibody antigen reaction readable was already there pre booster in the higher dilutions, while there was more antigen available for reaction in the testing at the lower dilutions? Hope my meaning is clear. ...

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