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Dictionary » V » V8 protease

V8 protease

Definition

noun

A serine protease isolated from Staphylococcus aureus strain V8, capable of cleaving peptide bonds on the carboxyl side of glutamate and aspartate residues in a sodium phosphate buffer


Supplement

V8 protease released by Staphylococcus aureus V8 strain improves the latter's pathogenicity. It acts by cleaving and inactivating antibodies and other antimicrobial peptides.

V8 protease can also be used as cleavage enzyme in research methods (e.g. amino acid sequence determination or peptide mapping).


Also called:

  • Glu-C (EC 3.4.21.9)

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Relationship between total protein and enzyme activity

Just for some reason more lytic enzymes do not help with more activity. Especially if you add protease, your enzyme will be probably degraded.

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by JackBean
Mon Sep 03, 2012 8:41 am
 
Forum: Molecular Biology
Topic: Relationship between total protein and enzyme activity
Replies: 1
Views: 2371

Relationship between total protein and enzyme activity

... fungus Aspergillus Oryzae.The lytic enzymatic cocktail was Yatalase, which composing of Chitinase, Chitobiase, Chitosanase, beta-1,3-glucanase and Protease. I used the cocktail (10 mg/ml) to lyse 5, 10, 20, 30, 40, 50, & 60% w/v of fungal concentration. After incubation time (60 mins), the ...

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by schroddycat
Sun Sep 02, 2012 12:34 pm
 
Forum: Molecular Biology
Topic: Relationship between total protein and enzyme activity
Replies: 1
Views: 2371

identifying 2 unknown bacterias

I have a gram negative, rod positive capsule present negative amylase reaction acidic, curd formation, peptonization in the protease reaction positive catalase reaction negative H2S reaction negative motility positive indole reaction positive nitrate reduction acidic, gas production ...

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by jl930
Wed Jul 18, 2012 4:57 am
 
Forum: Microbiology
Topic: identifying 2 unknown bacterias
Replies: 1
Views: 1699

Lowry method's wavelength choice

dear users, I'm on my research about bacterial protease isolation and assay now. I'm about to take an assay to total protein content by Lowry method. I'm searching the best protocol for the method, that is, I found the assay's wavelegth usually ...

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by faredeiss
Tue Jul 10, 2012 4:17 pm
 
Forum: Molecular Biology
Topic: Lowry method's wavelength choice
Replies: 1
Views: 842

Amyloid beta (A4) precursor protein degradation products

... with different length of the peptide chain isoforms, APP695, APP751 and APP770. Compared with the other two isoforms, APP695 lacks the Kunitz protease inhibitor (KPI) region outside the cell. Neurons expressing APP695 subtypes in the cerebral cortex of APP695, APP751 and APP770 mRNA ratio ...

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by Anonymous
Tue Jun 12, 2012 3:11 am
 
Forum: General Discussion
Topic: Amyloid beta (A4) precursor protein degradation products
Replies: 1
Views: 3081
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