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V8 protease

Definition

noun

A serine protease isolated from Staphylococcus aureus strain V8, capable of cleaving peptide bonds on the carboxyl side of glutamate and aspartate residues in a sodium phosphate buffer


Supplement

V8 protease released by Staphylococcus aureus V8 strain improves the latter's pathogenicity. It acts by cleaving and inactivating antibodies and other antimicrobial peptides.

V8 protease can also be used as cleavage enzyme in research methods (e.g. amino acid sequence determination or peptide mapping).


Also called:

  • Glu-C (EC 3.4.21.9)

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Fishing the upstream protease of a protein

Hi deal all, Recently I'd found the protein I'm working on was cleaved to a shorter form after being phosphorylated by Src. Anyone has good suggestion how to identify the possible upstream cutter? Thanks in advance (:

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by kingsupercat
Wed Nov 20, 2013 6:04 am
 
Forum: Cell Biology
Topic: Fishing the upstream protease of a protein
Replies: 0
Views: 637

Help with unknown

... Oxidase/Kovac's - EMB plate showed a maroonish color Mannitol and gas + Glucose and gas + Lactose - Phenylalanine - Nitrate to nitrite + Amylase - Protease for casein (Unsure) Gelatin - Urea - Thanks in advance!!

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by kayleighat
Sun Oct 20, 2013 2:43 am
 
Forum: Microbiology
Topic: Help with unknown
Replies: 0
Views: 2576

Relationship between total protein and enzyme activity

Just for some reason more lytic enzymes do not help with more activity. Especially if you add protease, your enzyme will be probably degraded.

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by JackBean
Mon Sep 03, 2012 8:41 am
 
Forum: Molecular Biology
Topic: Relationship between total protein and enzyme activity
Replies: 1
Views: 4700

Relationship between total protein and enzyme activity

... fungus Aspergillus Oryzae.The lytic enzymatic cocktail was Yatalase, which composing of Chitinase, Chitobiase, Chitosanase, beta-1,3-glucanase and Protease. I used the cocktail (10 mg/ml) to lyse 5, 10, 20, 30, 40, 50, & 60% w/v of fungal concentration. After incubation time (60 mins), the ...

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by schroddycat
Sun Sep 02, 2012 12:34 pm
 
Forum: Molecular Biology
Topic: Relationship between total protein and enzyme activity
Replies: 1
Views: 4700

identifying 2 unknown bacterias

I have a gram negative, rod positive capsule present negative amylase reaction acidic, curd formation, peptonization in the protease reaction positive catalase reaction negative H2S reaction negative motility positive indole reaction positive nitrate reduction acidic, gas production ...

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by jl930
Wed Jul 18, 2012 4:57 am
 
Forum: Microbiology
Topic: identifying 2 unknown bacterias
Replies: 1
Views: 3135
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