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V2 protease

V2 protease

(Science: enzyme) isolated from virulent strain of dichelobacter nodosus; differs from b2 protease by a single amino acid at position 219; genbank l38395

Registry number: EC 3.4.21.-

Synonym: aprv2 gene product, dn aprv2 gene product


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use of a bacterial protease inhibitor cocktail with mammalia

Dear fellow biochemists, I need some advice on Western Blotting, more specifically the use of certain protease inhibitors with the RIPA cell lysis buffer and protease inhibitor cocktail. A Millipore technical representative recommended my using "539134 | Protease Inhibitor ...

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by rpwdoc
Tue Aug 26, 2014 10:08 pm
 
Forum: Cell Biology
Topic: use of a bacterial protease inhibitor cocktail with mammalia
Replies: 0
Views: 398

Fishing the upstream protease of a protein

Hi deal all, Recently I'd found the protein I'm working on was cleaved to a shorter form after being phosphorylated by Src. Anyone has good suggestion how to identify the possible upstream cutter? Thanks in advance (:

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by kingsupercat
Wed Nov 20, 2013 6:04 am
 
Forum: Cell Biology
Topic: Fishing the upstream protease of a protein
Replies: 0
Views: 697

Help with unknown

... Oxidase/Kovac's - EMB plate showed a maroonish color Mannitol and gas + Glucose and gas + Lactose - Phenylalanine - Nitrate to nitrite + Amylase - Protease for casein (Unsure) Gelatin - Urea - Thanks in advance!!

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by kayleighat
Sun Oct 20, 2013 2:43 am
 
Forum: Microbiology
Topic: Help with unknown
Replies: 0
Views: 2837

Relationship between total protein and enzyme activity

Just for some reason more lytic enzymes do not help with more activity. Especially if you add protease, your enzyme will be probably degraded.

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by JackBean
Mon Sep 03, 2012 8:41 am
 
Forum: Molecular Biology
Topic: Relationship between total protein and enzyme activity
Replies: 1
Views: 5008

Relationship between total protein and enzyme activity

... fungus Aspergillus Oryzae.The lytic enzymatic cocktail was Yatalase, which composing of Chitinase, Chitobiase, Chitosanase, beta-1,3-glucanase and Protease. I used the cocktail (10 mg/ml) to lyse 5, 10, 20, 30, 40, 50, & 60% w/v of fungal concentration. After incubation time (60 mins), the ...

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by schroddycat
Sun Sep 02, 2012 12:34 pm
 
Forum: Molecular Biology
Topic: Relationship between total protein and enzyme activity
Replies: 1
Views: 5008
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