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Upstream

upstream

(Science: molecular biology) refers to nucleotide sequences that precede the codons specifying the mRNA or that precede (are on the 5' side of) the protein coding sequence. Also used of the early events in any process that involves sequential reactions.


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Fishing the upstream protease of a protein

... protein I'm working on was cleaved to a shorter form after being phosphorylated by Src. Anyone has good suggestion how to identify the possible upstream cutter? Thanks in advance (:

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by kingsupercat
Wed Nov 20, 2013 6:04 am
 
Forum: Cell Biology
Topic: Fishing the upstream protease of a protein
Replies: 0
Views: 665

Re: Markerless mutants using suicide vector

... DNA without some selectable marker, we'll just have a difficult time screening for our mutant. One interesting detail: the GC content of the upstream and downstream flanking regions used to recombine with the flanking regions of our gene of interest for deletion is quite different. The upstream ...

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by dhkwak1
Wed Dec 19, 2012 1:04 am
 
Forum: Genetics
Topic: Markerless mutants using suicide vector
Replies: 2
Views: 2668

Help on biology question?

3,Imagine you had these two strains of drosophila: AA= contains the gene for Gal 4 and a UAS upstream of alcohol dehydrogenase BB= has a UAS upstream of Beta- Galactosidase What would you expect to be able to detect in the flies from the following crosses: AA X AA Options: ...

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by revision
Tue Dec 11, 2012 6:38 pm
 
Forum: Molecular Biology
Topic: Help on biology question?
Replies: 0
Views: 1155

mRNA transcripts, cis/trans-acting questions

... than GAL2) in the galactose metabolism pathway are transcribed normally in this mutant. ______ e. The DNA sequence region containing the UAS sites upstream of GAL2 is deleted in this mutant. You are studying the genetics of a negatively regulated operon in E. coli, in which a single mRNA transcript ...

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by animus31
Fri Nov 09, 2012 11:58 am
 
Forum: Genetics
Topic: mRNA transcripts, cis/trans-acting questions
Replies: 0
Views: 2087

A question I'm finding hard to answer (recombinant protein)

... promoter sequence should be selected that is optimum for the desired expression levels and this must be cloned into the expression vector upstream of the foreign gene to be expressed. 2- Eukaryotic genes often contain introns. Bacteria do not have the splicing machinery to splice these ...

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by ibz52002
Sat Apr 28, 2012 4:45 pm
 
Forum: Cell Biology
Topic: A question I'm finding hard to answer (recombinant protein)
Replies: 9
Views: 6766
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