Upstream

... DNA without some selectable marker, we'll just have a difficult time screening for our mutant. One interesting detail: the GC content of the upstream and downstream flanking regions used to recombine with the flanking regions of our gene of interest for deletion is quite different. The upstream ...

See entire post

3,Imagine you had these two strains of drosophila: AA= contains the gene for Gal 4 and a UAS upstream of alcohol dehydrogenase BB= has a UAS upstream of Beta- Galactosidase What would you expect to be able to detect in the flies from the following crosses: AA X AA Options: ...

See entire post

... than GAL2) in the galactose metabolism pathway are transcribed normally in this mutant. ______ e. The DNA sequence region containing the UAS sites upstream of GAL2 is deleted in this mutant. You are studying the genetics of a negatively regulated operon in E. coli, in which a single mRNA transcript ...

See entire post

... promoter sequence should be selected that is optimum for the desired expression levels and this must be cloned into the expression vector upstream of the foreign gene to be expressed. 2- Eukaryotic genes often contain introns. Bacteria do not have the splicing machinery to splice these ...

See entire post

... that help in transcription which eventually will lead to produce proteins, the core promoter. This is a sequence of dna bases which are located upstream of about -35 bases( ie. the opposite direction of the transcription reation). They help in aid of the transcription to happen smoothly by having ...

See entire post