Ung

... some experiments with a "blocking oligo" without success. The second reason was that I only thought of having U-bases and digest it with UNG excision mix, and that is the option that is out of the question (it will also digest something that I don't want digested). The method you suggest ...

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... good pipetting techique). If the problem is common, convince your boss to invest in a mix containing dUTP and start treating all reactions with UNG.

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