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Ultrafiltration

Definition

noun

(1) A high pressure filtration through a semipermeable membrane in which colloidal particles are retained while the small sized solutes and the solvent are forced to move across the membrane by hydrostatic pressure forces.

(2) The process of separating particles of one size from particles of another size in a suspending medium using a filter (of particular pore size), especially used in industry and scientific research for purifying and concentrating macromolecular (103 - 106 Da) solutions.


Supplement

Ultrafiltration is a vital process that takes place in the kidneys. With ultrafiltration, large molecules (such as cells and proteins) that are essential are separated or filtered out to be reabsorbed. The ultrafiltrate, in turn, is passed through the proximal tubule, the loop of Henle, the distal convoluted tubule, and a series of collecting ducts to form urine.


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inventing basic lab protocols

... in lab) 2) Basics in biochem lab (weigthing, pipetting, buffers etc) 3) Extraction of proteins, precipitations, centrifugation, dialysis and ultrafiltration 4) Extraktion, filtration, sublimation (coffein extraction from tea) 5) Spectrofotometric (determination of Ni2+, protein by Bradford's, ...

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by JackBean
Wed Apr 20, 2011 3:40 pm
 
Forum: General Discussion
Topic: inventing basic lab protocols
Replies: 0
Views: 1408

Re: Cellulose filater papers and Xylanases?

Hi JackBean, Thanks for the reply. I am using regenerated cellulose membranes in the concentrating device. I do use PES but in ultrafiltration units. Now dilemma in choosing which one is perfect for xylanases, coz, the amount of sample is small.

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by Microbio
Tue Oct 06, 2009 7:18 am
 
Forum: General Discussion
Topic: Cellulose filater papers and Xylanases?
Replies: 3
Views: 1113

The Kidney

... I have just posted up a website (with an odd name), concerning the kidney. The website is meant to help GCSE students concerning the topics of Ultrafiltration, Osmoregulation and Selective Re-absorption. The website itself will be changed later on to look more professional, and have an easy ...

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by jsmith613
Sat Jul 25, 2009 9:44 pm
 
Forum: Human Biology
Topic: The Kidney
Replies: 5
Views: 5570

Re: Fractination of SA into Cytosol., Cell Wall, Cytoplasmic

... cells (5-6K rpm/ up to 30 min/4C) 3. collect the supernatant containing extracellular proteins, you may need to concentrate them (AmSO4/memebrane ultrafiltration: vivaspin, centricon, whatever else 10 K cut-off) and store at 4C 4. wash the cells thoroughly with fresh media /PBS/whatever you have ...

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by Ewa
Wed Jul 04, 2007 6:23 pm
 
Forum: Microbiology
Topic: Fractination of SA into Cytosol., Cell Wall, Cytoplasmic
Replies: 3
Views: 5105

protien molecules

Research ultrafiltration

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by James
Fri Feb 16, 2007 4:18 pm
 
Forum: Human Biology
Topic: protien molecules
Replies: 3
Views: 1930
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