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Ultracentrifuge

A high speed centrifuge used to determine the relative molecular masses of large molecules in high polymers and proteins.Subject to the action of an ultracentrigue.A type of centrifuge capable of spinning at 500 000 rpm, that is half a million revolutions per minute, which is used to separate tiny particles from one another due to the force involved.


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altering centrifuge procedure

... thanks a million canalon. By the way, since I will not use some high speed, I assume usual centrifuge with 14000rpm can do it right? Or the use of ultracentrifuge is compulsory?

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by adihutama
Wed Mar 02, 2011 12:36 am
 
Forum: Molecular Biology
Topic: altering centrifuge procedure
Replies: 3
Views: 2096

altering centrifuge procedure

... the forum about this, and it seems that no topic has discussed it yet. Here's how it goes: I m trying to do microsome isolation that involve ultracentrifuge @ 25000 xg for 15 min. The problem is, although we do have an ultracentrifuge, it cant reach 25000 xg. I know this sound ridiculous, ...

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by adihutama
Tue Mar 01, 2011 4:37 am
 
Forum: Molecular Biology
Topic: altering centrifuge procedure
Replies: 3
Views: 2096

semester long projects any suggestions????

... Disposal facilities UV-VIS scanning spectrophotometers IR spectrophotometers Atomic Absorption Spectrophotometer NMR spectrophotometer HPLC Ultracentrifuge High speed vaccum concentrator

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by justcurious91
Thu Jun 17, 2010 6:00 pm
 
Forum: General Discussion
Topic: semester long projects any suggestions????
Replies: 3
Views: 3318

Fractination of SA into Cytosol., Cell Wall, Cytoplasmic

... correctly, but it sounds tricky to me. I can't see how you're going to do this without any centrifuge, but perhaps you don't need a full-blown ultracentrifuge. If you have access to either a benchtop Eppendorf centrifuge, which can spin a 1.5 ml tube up to 17,000xg, or an RC-5Plus or equivalent ...

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by blcr11
Mon Jun 04, 2007 4:50 pm
 
Forum: Microbiology
Topic: Fractination of SA into Cytosol., Cell Wall, Cytoplasmic
Replies: 3
Views: 4992

Fractination of SA into Cytosol., Cell Wall, Cytoplasmic

... S. aureus. I just control with Western Blot. The problem is, i can't find a protocol for isolation or digestion of these fractions. We have no ultracentrifuge! I would be very happy for helping... Best regards Ilknur

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by RuNkLi
Mon Jun 04, 2007 2:39 pm
 
Forum: Microbiology
Topic: Fractination of SA into Cytosol., Cell Wall, Cytoplasmic
Replies: 3
Views: 4992
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